Adipose tissue inflammation is slightly increased in macrophage CD40 deficient mice without resulting in altered cytokine release. Suzanne A. B. M. Aarts Myrthe E. Reiche Myrthe den Toom Linda Beckers Marion J. J. Gijbels Norbert Gerdes Menno P. J. de Winther Esther Lutgens 10.1371/journal.pone.0202150.g004 https://plos.figshare.com/articles/figure/Adipose_tissue_inflammation_is_slightly_increased_in_macrophage_CD40_deficient_mice_without_resulting_in_altered_cytokine_release_/6956870 <p><b>(A)</b> Quantification of CD45 staining for leukocytes of EpAT (n = 16 for HFD and n = 7 for SFD). <b>(B)</b> Quantification of Mac3 staining for macrophages in EpAT (n = 16 for HFD and n = 7 for SFD). <b>(C)</b> Quantification of CD3<sup>+</sup> staining for T cells in EpAT (n = 16 for HFD and n = 7 for SFD). <b>(D)</b> Flow cytometric analysis of leukocyte subsets in EpAT, as % of total CD45<sup>+</sup> leukocytes (n = 7-9/group). <b>(E)</b> mRNA expression of inflammatory markers in EpAT (n = 7-9/group). Data is presented as mean ± SEM.</p> 2018-08-10 17:39:43 adipose tissue leukocyte infiltration WT 40L Macrophage CD 40 SFD adipose tissue inflammation 40-CD ameliorates adipose tissue inflammation insulin resistance HFD CD 40 flfl LysM cre increases insulin sensitivity macrophage CD 40 adipose tissue weight DIO plasma adiponectin levels triglyceride levels macrophage CD 40. CD 40 flfl LysM cre mice adipose tissue cytokine levels