Sanvitale, Caroline E. Kerr, Georgina Chaikuad, Apirat Ramel, Marie-Christine H. Mohedas, Agustin Reichert, Sabine Wang, You Triffitt, James T. Cuny, Gregory D. Yu, Paul B. S. Hill, Caroline Bullock, Alex N. K02288 induces dorsalization of zebrafish embryos. <p>(A) Brightfield photographs of 26 hours old <i>Tg(BRE:mRFP)</i> transgenic embryos treated with DMSO or varying doses of K02288 from the 8- to 16-cell stage. Severity of the dorsalization correlated with the dose of K02288. Very strong dorsalized phenotypes were observed with 8–10 µM K02288. (B) The phenotypes of the embryos shown in A were classified according to Kishimoto et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062721#pone.0062721-Kishimoto1" target="_blank">[49]</a>. (C) Western blot for mRFP in extracts prepared from <i>Tg(BRE:mRFP)</i> embryos treated in a parallel experiment. Loss of mRFP protein was evident at 8–10 µM K02288. As a control, the effects of dorsomorphin (DM) and LDN-193189 (LDN) on mRFP expression are also shown. Protein loading control is shown with the MCM6 blot.</p> Biochemistry;chemical biology;drug discovery;proteins;Small molecules;biophysics;Biomacromolecule-ligand interactions;Protein chemistry;developmental biology;morphogenesis;Molecular cell biology;Signal transduction;Signaling cascades;Protein kinase signaling cascade;TGF-beta signaling cascade;Membrane receptor signaling;Signaling pathways;medicinal chemistry;dorsalization;zebrafish 2013-04-30
    https://plos.figshare.com/articles/figure/_K02288_induces_dorsalization_of_zebrafish_embryos_/694799
10.1371/journal.pone.0062721.g005