10.1371/journal.pone.0062721.g005
Caroline E. Sanvitale
Caroline E.
Sanvitale
Georgina Kerr
Georgina
Kerr
Apirat Chaikuad
Apirat
Chaikuad
Marie-Christine Ramel
Marie-Christine
Ramel
Agustin H. Mohedas
Agustin
H. Mohedas
Sabine Reichert
Sabine
Reichert
You Wang
You
Wang
James T. Triffitt
James T.
Triffitt
Gregory D. Cuny
Gregory D.
Cuny
Paul B. Yu
Paul B.
Yu
Caroline S. Hill
Caroline
S. Hill
Alex N. Bullock
Alex N.
Bullock
K02288 induces dorsalization of zebrafish embryos.
Public Library of Science
2013
Biochemistry
chemical biology
drug discovery
proteins
Small molecules
biophysics
Biomacromolecule-ligand interactions
Protein chemistry
developmental biology
morphogenesis
Molecular cell biology
Signal transduction
Signaling cascades
Protein kinase signaling cascade
TGF-beta signaling cascade
Membrane receptor signaling
Signaling pathways
medicinal chemistry
dorsalization
zebrafish
2013-04-30 01:19:59
Figure
https://plos.figshare.com/articles/figure/_K02288_induces_dorsalization_of_zebrafish_embryos_/694799
<p>(A) Brightfield photographs of 26 hours old <i>Tg(BRE:mRFP)</i> transgenic embryos treated with DMSO or varying doses of K02288 from the 8- to 16-cell stage. Severity of the dorsalization correlated with the dose of K02288. Very strong dorsalized phenotypes were observed with 8–10 µM K02288. (B) The phenotypes of the embryos shown in A were classified according to Kishimoto et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062721#pone.0062721-Kishimoto1" target="_blank">[49]</a>. (C) Western blot for mRFP in extracts prepared from <i>Tg(BRE:mRFP)</i> embryos treated in a parallel experiment. Loss of mRFP protein was evident at 8–10 µM K02288. As a control, the effects of dorsomorphin (DM) and LDN-193189 (LDN) on mRFP expression are also shown. Protein loading control is shown with the MCM6 blot.</p>