10.1371/journal.pone.0062721.g005 Caroline E. Sanvitale Caroline E. Sanvitale Georgina Kerr Georgina Kerr Apirat Chaikuad Apirat Chaikuad Marie-Christine Ramel Marie-Christine Ramel Agustin H. Mohedas Agustin H. Mohedas Sabine Reichert Sabine Reichert You Wang You Wang James T. Triffitt James T. Triffitt Gregory D. Cuny Gregory D. Cuny Paul B. Yu Paul B. Yu Caroline S. Hill Caroline S. Hill Alex N. Bullock Alex N. Bullock K02288 induces dorsalization of zebrafish embryos. Public Library of Science 2013 Biochemistry chemical biology drug discovery proteins Small molecules biophysics Biomacromolecule-ligand interactions Protein chemistry developmental biology morphogenesis Molecular cell biology Signal transduction Signaling cascades Protein kinase signaling cascade TGF-beta signaling cascade Membrane receptor signaling Signaling pathways medicinal chemistry dorsalization zebrafish 2013-04-30 01:19:59 Figure https://plos.figshare.com/articles/figure/_K02288_induces_dorsalization_of_zebrafish_embryos_/694799 <p>(A) Brightfield photographs of 26 hours old <i>Tg(BRE:mRFP)</i> transgenic embryos treated with DMSO or varying doses of K02288 from the 8- to 16-cell stage. Severity of the dorsalization correlated with the dose of K02288. Very strong dorsalized phenotypes were observed with 8–10 µM K02288. (B) The phenotypes of the embryos shown in A were classified according to Kishimoto et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062721#pone.0062721-Kishimoto1" target="_blank">[49]</a>. (C) Western blot for mRFP in extracts prepared from <i>Tg(BRE:mRFP)</i> embryos treated in a parallel experiment. Loss of mRFP protein was evident at 8–10 µM K02288. As a control, the effects of dorsomorphin (DM) and LDN-193189 (LDN) on mRFP expression are also shown. Protein loading control is shown with the MCM6 blot.</p>