10.1371/journal.pone.0063038.g008 Leonardo J. Leon Leonardo J. Leon Nagarekha Pasupuleti Nagarekha Pasupuleti Fredric Gorin Fredric Gorin Kermit L. Carraway III Kermit L. Carraway III UCD38B does not induce calcium-dependent necrosis. Public Library of Science 2013 Biochemistry drug discovery Small molecules Molecular cell biology Cell death Cell growth Cellular stress responses Obstetrics and gynecology breast cancer oncology Cancer treatment Chemotherapy and drug treatment Cancers and neoplasms Breast tumors induce calcium-dependent 2013-04-30 01:19:55 Figure https://plos.figshare.com/articles/figure/_UCD38B_does_not_induce_calcium_dependent_necrosis_/694795 <p>(<b>A</b>) LDH cell death assays were carried out with MDA-MB-231 cells that were treated with UCD38B and ionomycin (IM) for 24 hours with and without 1 hour pre-treatment with 10 µM BAPTA-AM. (<b>B</b>) Cell viability after 24 hours was measured in the absence and presence of 25 µM RIP1 necroptosis inhibitor necrostatin-1 using the MTT assay. (<b>C</b>) LDH cell death assays were carried out with cells treated with 8 µM shikonin or 250 µM UCD38B in the presence and absence of 25 µM necrostatin-1. Results depicted in each panel are representative of three independent experiments. *, <i>P<0.05</i>; **, <i>P</i><0.001.</p>