10.1371/journal.pone.0061210.g001
Teresa J. Broering
Teresa
J. Broering
Hongyan Wang
Hongyan
Wang
Naomi K. Boatright
Naomi
K. Boatright
Yang Wang
Yang
Wang
Katherine Baptista
Katherine
Baptista
Gilda Shayan
Gilda
Shayan
Kerry A. Garrity
Kerry
A. Garrity
Can Kayatekin
Can
Kayatekin
Daryl A. Bosco
Daryl
A. Bosco
C. Robert Matthews
C. Robert
Matthews
Donna M. Ambrosino
Donna
M. Ambrosino
Zuoshang Xu
Zuoshang
Xu
Gregory J. Babcock
Gregory
J. Babcock
HuMabs recognize different regions of the hSOD1 protein.
Public Library of Science
2013
Biochemistry
proteins
protein structure
drug discovery
biotechnology
immunology
immunoglobulins
neurology
Motor neuron diseases
amyotrophic lateral sclerosis
Neurodegenerative diseases
Neuromuscular diseases
regions
hsod1
2013-04-17 01:18:20
Figure
https://plos.figshare.com/articles/figure/_HuMabs_recognize_different_regions_of_the_hSOD1_protein_/684700
<p>(A) Full-length hSOD1 (amino acids 1–153) and various portions of the protein were expressed in bacteria fused to the carboxy-terminus (C-terminus) of thioredoxin (Trx) and containing a C-terminal 6-histidine tag used for purification (Trx-hSOD1-WT-his). Each protein is represented in the figure as a hashed line with the beginning and ending amino acid number listed below the line. The proteins were coated on ELISA plates and binding of HuMabs (listed at the top right) detected with goat-anti-human antibody conjugated to alkaline phosphatase followed by PNPP substrate addition. ELISA results are listed to the right of the schematic; positive recognition is indicated by a plus sign while signals equivalent to background are indicated by a minus sign. HuMabs recognizing only full-length hSOD1 were designated conformation dependent and are noted below with a C. HuMabs with an epitope that mapped to a linear sequence of amino acids are noted below with an L. (B) Minimal linear epitopes were determined with amino-terminal biotin-labeled overlapping peptides coated on streptavidin ELISA plates. Binding of HuMabs was assessed as described in A. The epitopes are noted as a grey box for each linear-epitope HuMab with the amino acids (aa) bound indicated. To distinguish these epitopes throughout the rest of the manuscript each linear-epitope HuMab has a subscripted L for linear followed by the initial amino acid of the epitope.</p>