Standard curve obtained by plotting the Ct against serial 10-fold dilutions of <i>L</i>. <i>donovani</i> DNA, starting at 10<sup>6</sup> parasites per reaction. TiwaryPuja KumarDinesh MishraMukesh Pratap SinghRudra RaiMadhukar SundarShyam 2013 <p>Ct represents the fractional cycle number reflecting a positive PCR result differentiated from background.</p>