10.1371/journal.pone.0199896.g006 Monica Boffito Monica Boffito Franca Di Meglio Franca Di Meglio Pamela Mozetic Pamela Mozetic Sara Maria Giannitelli Sara Maria Giannitelli Irene Carmagnola Irene Carmagnola Clotilde Castaldo Clotilde Castaldo Daria Nurzynska Daria Nurzynska Anna Maria Sacco Anna Maria Sacco Rita Miraglia Rita Miraglia Stefania Montagnani Stefania Montagnani Nicoletta Vitale Nicoletta Vitale Mara Brancaccio Mara Brancaccio Guido Tarone Guido Tarone Francesco Basoli Francesco Basoli Alberto Rainer Alberto Rainer Marcella Trombetta Marcella Trombetta Gianluca Ciardelli Gianluca Ciardelli Valeria Chiono Valeria Chiono Results from <i>in vivo</i> tests carried out in mice: Histological analysis and quantification of blood vessels in the tissues surrounding the implants. Public Library of Science 2018 nitrogen percentage increase Scaffolds subcutaneously CPC behavior Surface functionalization ELISA assay LN 1 surface PU scaffolds cells Scaffolds control functionalizing molecule C 1s core-level spectra non-cytotoxic residues polyurethane scaffolds contact angle measurements show changes host tissue blood vessel density CPC adhesion square-grid scaffolds regenerative medicine heart regeneration acrylic acid surface muscle cells heart formation niche extracellular matrix component plasma treatment carbodiimide chemistry PU-LN 1 PU-LN 1 scaffolds show LN 1 functionalization melt-extrusion additive manufacturing LN 1 PU-LN 1 scaffolds PU-G progenitor cells differentiation markers cell-adhesion protein O 1s X-ray photoelectron spectroscopy analysis 2018-07-06 17:41:34 Figure https://plos.figshare.com/articles/figure/Results_from_i_in_vivo_i_tests_carried_out_in_mice_Histological_analysis_and_quantification_of_blood_vessels_in_the_tissues_surrounding_the_implants_/6769955 <p>Histological analysis by hematoxylin and eosin staining of PU and PU-LN1 scaffolds and surrounding tissues explanted 15 <b>(A)</b> and 30 <b>(B)</b> days following subcutaneous implantation in mice. Arrows indicate blood vessels (scale bar: 50 μm). <b>(C)</b> Number of blood vessels per mm<sup>2</sup> in the tissues surrounding PU and PU-LN1 scaffolds, and explanted 15 and 30 days after subcutaneous implantation in mice. Unpaired two-tailed <i>t</i> test was used for statistical analysis.</p>