Detecting DEC pathogens in clinical samples. Uffe Vest Schneider Nikolaj Dam Mikkelsen Flemming Scheutz Alice Friis-Møller Gorm Lisby 10.1371/journal.pone.0199766.g005 https://plos.figshare.com/articles/figure/Detecting_DEC_pathogens_in_clinical_samples_/6685556 <p>The samples are reported as being either positive by both methods or positive by only one of the methods. All samples that were positive only by the Cliffhanger method were verified by Sanger sequencing.</p> 2018-06-26 17:36:12 Cliffhanger primers multiplex PCR efficacy antiparallel duplex DNA helix crude lysates diarrhoeagenic Escherichia coli multiplex PCR multiplexed PCR assay single-stranded DNA overhangs sample turn-around time fragment length separation Cliffhanger method faecal samples