10.1371/journal.pone.0058358.g002
Federico Gabrielli
Federico
Gabrielli
Roberto Salvi
Roberto
Salvi
Chiara Garulli
Chiara
Garulli
Cristina Kalogris
Cristina
Kalogris
Serena Arima
Serena
Arima
Luca Tardella
Luca
Tardella
Paolo Monaci
Paolo
Monaci
Serenella M. Pupa
Serenella
M. Pupa
Elda Tagliabue
Elda
Tagliabue
Maura Montani
Maura
Montani
Elena Quaglino
Elena
Quaglino
Lorenzo Stramucci
Lorenzo
Stramucci
Claudia Curcio
Claudia
Curcio
Cristina Marchini
Cristina
Marchini
Augusto Amici
Augusto
Amici
Identification of relevant conformational epitopes on HER2 oncoprotein.
Public Library of Science
2013
conformational
epitopes
her2
2013-03-29 12:57:22
Figure
https://plos.figshare.com/articles/figure/_Identification_of_relevant_conformational_epitopes_on_HER2_oncoprotein_/662336
<p>Screening of sera from metastatic and early breast cancer patients using an ELISA assay based on human LFPD (a). Sera from metastatic breast cancer patients recognized hum1, hum7, and hum11 fragments (blue lines). Sera from early breast cancer patients recognized hum1, hum7, hum9 and hum11 fragments (red lines). Green lines represent sera from healthy donor patients. Screening of sera from mice vaccinated with EC-TM plasmid by an ELISA assay based on rat LFPD (b). Both sera from non-tolerant Balb/c (blue line) and tolerant Balb/neuT (red line) mice recognized rat1, rat2, rat9 and rat11 conformational epitopes, although tolerant mice sera showed a lower reactivity. Sera from mice vaccinated with a truncated form of HER2 (EC4) recognized rat6, rat9 and rat11 fragments (orange line). Pepito based analysis of human HER2 (c). Pepito based analysis of rat HER2 (d). Red zones indicate the maximum probability to find a conformational epitope.</p>