10.1371/journal.pone.0060297.g004
Shinya Okamoto
Shinya
Okamoto
Yuanyuan Jiang
Yuanyuan
Jiang
Kiyoko Kawamura
Kiyoko
Kawamura
Masato Shingyoji
Masato
Shingyoji
Toshihiko Fukamachi
Toshihiko
Fukamachi
Yuji Tada
Yuji
Tada
Yuichi Takiguchi
Yuichi
Takiguchi
Koichiro Tatsumi
Koichiro
Tatsumi
Hideaki Shimada
Hideaki
Shimada
Kenzo Hiroshima
Kenzo
Hiroshima
Hiroshi Kobayashi
Hiroshi
Kobayashi
Masatoshi Tagawa
Masatoshi
Tagawa
ZOL-induced up-regulation of p53 and knockdown of the p53 expressions with siRNA.
Public Library of Science
2013
up-regulation
p53
knockdown
expressions
2013-03-29 09:59:51
Figure
https://plos.figshare.com/articles/figure/_ZOL_induced_up_regulation_of_p53_and_knockdown_of_the_p53_expressions_with_siRNA_/661994
<p>(A, B) CDDP-treated (20 µM) and ZOL-treated (48 h) cells were subjected to Western blot analysis and probed with antibodies as indicated. Actin was used as a loading control. (C) Cells were treated with CDDP and/or ZOL for 48 h at the indicated concentrations and the expression levels of phosphorylated p53 were examined. (D) Cells were transfected with p53-targeted siRNA (p53-siRNA) or non-targeted control siRNA (Control) for 24 h and then treated with ZOL (50 µM) for 48 h. The lysate was subjected to Western blot analysis. (E) Cells were transfected with siRNA as indicted and were treated with ZOL for 3 days. The cell viabilities were measured with the WST assay and means of triplicated samples with the SD bars are shown. (F) Flow cytometrical analyses of MSTO-211H cells that were transfected with respective siRNA for 24 h and then treated with ZOL (50 µM) for 48 h. (G, H) Cells transfected with p53-siRNA were treated with different doses of ZOL and CDDP as indicated for 3 days and the CI values based on the cell viabilities were calculated at different Fa points with CalcuSyn software.</p>