10.1371/journal.pone.0058857.g009 Aseervatham Anusha Amali Aseervatham Anusha Amali Lawrence Sie Lawrence Sie Christoph Winkler Christoph Winkler Mark Featherstone Mark Featherstone Expression of <i>meis1.1,</i> but not <i>cdx4</i>, is impaired in <i>hoxd4a</i> morphants at the shield stage. Public Library of Science 2013 findings place hoxd 4a cdx Hox genes ventral-most mesoderm fated hoxd 4a function group paralog Hoxd 4 display malformations 13 hours post fertilization meis 1.1 expression hemangioblast markers scl 1 hoxd 4a acts PLM orthologous hoxd 4a gene Zebrafish hoxd 4a Acts Upstream meis 1.1 2013-03-16 07:38:40 Figure https://plos.figshare.com/articles/figure/_Expression_of_meis1_1_but_not_cdx4_is_impaired_in_hoxd4a_morphants_at_the_shield_stage_/653067 <p>Expression of <i>cdx4</i> (A,B), <i>hoxd4a</i> (C–E) and <i>meis1.1</i> (F–H) in control (A,C,F), <i>hoxd4a</i> morphants (B,D,G) and rescuants injected with <i>hoxd4a</i> mRNA (E,H) observed at the shield stage. Asterisks denote the ventral-most mesoderm fated to give rise to hemangioblast in addition to unipotential hematopoietic and angiogenic progenitors. Ratios indicate the fraction of embryos showing the presented phenotype. Scale bars equal 100 µm. (I) qRT-PCR was used to quantitate relative mRNA levels in controls, morphants and rescuants as indicated. Error bars present the standard error. Decreased expression of <i>hoxd4a</i> and <i>meis1.1</i> in <i>hoxd4a</i> morphants is significantly lower than both controls and rescuants to p≤0.02.</p>