10.1371/journal.pone.0058857.g008 Aseervatham Anusha Amali Aseervatham Anusha Amali Lawrence Sie Lawrence Sie Christoph Winkler Christoph Winkler Mark Featherstone Mark Featherstone <i>meis1.1</i> is down regulated in <i>hoxd4a</i> morphants and <i>meis1.1</i> mRNA rescues hematopoietic and vasculogenic gene expression in <i>hoxd4a</i> morphants. Public Library of Science 2013 findings place hoxd 4a cdx Hox genes ventral-most mesoderm fated hoxd 4a function group paralog Hoxd 4 display malformations 13 hours post fertilization meis 1.1 expression hemangioblast markers scl 1 hoxd 4a acts PLM orthologous hoxd 4a gene Zebrafish hoxd 4a Acts Upstream meis 1.1 2013-03-16 07:37:39 Figure https://plos.figshare.com/articles/figure/_meis1_1_is_down_regulated_in_hoxd4a_morphants_and_meis1_1_mRNA_rescues_hematopoietic_and_vasculogenic_gene_expression_in_hoxd4a_morphants_/653063 <p>Expression of <i>meis1.1</i> in control (A), morphant (B) and <i>hoxd4a</i>-rescuant (C) embryos at 26–28 hpf. (D–I) At 13 hpf, normal expression of <i>gata1</i>, <i>scl1</i> and <i>fli1</i> (D–F) is reduced in <i>hoxd4a</i> morphants (G–I), but rescued upon co-injection with capped mRNA for <i>meis1.1</i> (J–L). (M,N) Rescue of vascular patterning in <i>hoxd4a</i> morphants by co-injection with capped mRNA for <i>meis1.1</i> as visualized in Tg(fli1:EGFP) transgenics at 48 hpf (M) and by alkaline phosphatase staining at 72 hpf (N). Scale bars equal 100 µm.</p>