Effect of borrelidin on the growth of WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> parasites.
Sanya Chadha
Ramachandran Vijayan
Sakshi Gupta
Manoj Munde
Samudrala Gourinath
Rentala Madhubala
10.1371/journal.pntd.0006575.g007
https://plos.figshare.com/articles/figure/Effect_of_borrelidin_on_the_growth_of_WT_i_ThrRS_NEO_i_and_i_ThrRS_NEO_pThrRS_i_sup_i_i_sup_i_i_parasites_/6512684
<p>(A) and (B) Inhibition profile of borrelidin for the promastigote (A) and intracellular amastigote (B) growth of WT parasites. Percentage parasite survival was plotted against different concentrations of borrelidin. (C) and (D) The leishmanicidal effect of borrelidin was checked on promastigotes (C) and amastigotes (D) of WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> parasites. The mean IC<sub>50</sub> values were calculated for borrelidin and plotted as bar graphs. (E) The effect of miltefosine on WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> promastigotes. The bar graphs represent mean ± SD with <i>n</i> = 3. * <i>p</i> < 0.05.</p>
2018-06-13 17:53:50
parasite
threonyl tRNA synthetase
target Background Aminoacyl tRNA synthetases
Methodology Recombinant Ld ThrRS
Ld ThrRS
aminoacylation activity
IC 50
Leishmania donovani threonyl tRNA synthetase
Conclusions Recombinant Ld ThrRS
r Ld ThrRS
drug discovery efforts