Effect of borrelidin on the growth of WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> parasites. Sanya Chadha Ramachandran Vijayan Sakshi Gupta Manoj Munde Samudrala Gourinath Rentala Madhubala 10.1371/journal.pntd.0006575.g007 https://plos.figshare.com/articles/figure/Effect_of_borrelidin_on_the_growth_of_WT_i_ThrRS_NEO_i_and_i_ThrRS_NEO_pThrRS_i_sup_i_i_sup_i_i_parasites_/6512684 <p>(A) and (B) Inhibition profile of borrelidin for the promastigote (A) and intracellular amastigote (B) growth of WT parasites. Percentage parasite survival was plotted against different concentrations of borrelidin. (C) and (D) The leishmanicidal effect of borrelidin was checked on promastigotes (C) and amastigotes (D) of WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> parasites. The mean IC<sub>50</sub> values were calculated for borrelidin and plotted as bar graphs. (E) The effect of miltefosine on WT, <i>ThrRS/NEO</i> and <i>ThrRS/NEO[pThrRS</i><sup><i>+</i></sup><i>]</i> promastigotes. The bar graphs represent mean ± SD with <i>n</i> = 3. * <i>p</i> < 0.05.</p> 2018-06-13 17:53:50 parasite threonyl tRNA synthetase target Background Aminoacyl tRNA synthetases Methodology Recombinant Ld ThrRS Ld ThrRS aminoacylation activity IC 50 Leishmania donovani threonyl tRNA synthetase Conclusions Recombinant Ld ThrRS r Ld ThrRS drug discovery efforts