%0 Figure %A L. Pfaff, Kathleen %A W. King, Randall %D 2013 %T Arginine residues in and near the D-box are required for cyclin B1 localization to mitotic chromosomes. %U https://plos.figshare.com/articles/figure/_Arginine_residues_in_and_near_the_D_box_are_required_for_cyclin_B1_localization_to_mitotic_chromosomes_/650186 %R 10.1371/journal.pone.0059169.g005 %2 https://plos.figshare.com/ndownloader/files/984587 %K residues %K d-box %K cyclin %K b1 %K localization %K mitotic %X

A. Representative images of mitotic BS-C-1 cells expressing cyclin B1 containing mutations in and near the D-box. White arrows indicate the position of the metaphase plate as seen in the DIC image. Scale bar = 10 µm. B. CER box-and-whisker plots for the constructs shown in part A. The red dotted line indicates the threshold of 1.6. N  = 10–14, * indicates p<0.05, ** indicates p<0.01 and *** indicated p<0.001 compared to WT1–433. Further details including mean CER, standard deviations, and Wilcoxon exact test p-values can be found in Table S2. C. Representative images of mitotic BS-C-1 cells expressing cyclin B1 fragments bearing R42A or Δ3–8/R42A double mutations. White arrows indicate the position of the metaphase plate as seen in the DIC image. Scale bar = 10 µm. D. CER box-and-whisker plots for the constructs shown in part C. The red dotted line indicates the threshold of 1.6. N = 9–13, * indicates p<0.05, ** indicated p<0.01 and ***indicates p<0.001 compared to WT counterpart. Further details including mean CER, standard deviations, and Wilcoxon exact test p-values can be found in Table S2.

%I PLOS ONE