10.1371/journal.pone.0198063.g002
Lorenzo Federico
Lorenzo
Federico
Liping Yang
Liping
Yang
Jason Brandon
Jason
Brandon
Manikandan Panchatcharam
Manikandan
Panchatcharam
Hongmei Ren
Hongmei
Ren
Paul Mueller
Paul
Mueller
Manjula Sunkara
Manjula
Sunkara
Diana Escalante-Alcalde
Diana
Escalante-Alcalde
Andrew J. Morris
Andrew J.
Morris
Susan S. Smyth
Susan
S. Smyth
Deficiency of adipose LPP3 does not impact body size or adipose tissue development in mice on a standard diet.
Public Library of Science
2018
adipocyte sphingolipid synthesis
diet-dependent sphingolipid synthesis
diet-induced adipose tissue expansion
adipocyte lipid metabolism
lipid phosphatase activity
LPP 3
LPP 3 substrate sphingosine 1- phosphate
serine palmitoyl transferase
intracellular PA-specific phosphatases
specificity membrane-associated lipid phosphate phosphatases
LPP 3 deficiency
Adipocyte LPP 3 deficiency
lipin 1 causes lipodystrophy
Lipid phosphate phosphatase 3
adipocyte LPP 3 deficiency
Western diet-fed mice
2018-06-11 17:31:24
Figure
https://plos.figshare.com/articles/figure/Deficiency_of_adipose_LPP3_does_not_impact_body_size_or_adipose_tissue_development_in_mice_on_a_standard_diet_/6482048
<p>Body weight of male (A) and female (B) <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open symbols) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed symbols) mice. Results are graphed as mean ± SD from 4–5 animals/group. Weight in grams of interscapular brown adipose tissue (IBAT), subcutaneous (SUB) white adipose tissue, and liver, and femur length (in mm) in 9-week old male (C) and female (D) <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open bars) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed bars) mice. Comparisons between genotypes were made by Students t-test. E. Representative histology of <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ) subcutaneous white adipose tissue stained with Periodic acid–Schiff (PAS) at 10x (left) and 20x (right) magnification. F. Expression analysis of inflammatory gene expression in visceral fat from <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open bars; n = 5) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed bars; n = 4) mice on high fat diet. Results are graphed as mean ± SD and data analyzed by two-tailed t-test.</p>