10.1371/journal.pone.0198063.g002 Lorenzo Federico Lorenzo Federico Liping Yang Liping Yang Jason Brandon Jason Brandon Manikandan Panchatcharam Manikandan Panchatcharam Hongmei Ren Hongmei Ren Paul Mueller Paul Mueller Manjula Sunkara Manjula Sunkara Diana Escalante-Alcalde Diana Escalante-Alcalde Andrew J. Morris Andrew J. Morris Susan S. Smyth Susan S. Smyth Deficiency of adipose LPP3 does not impact body size or adipose tissue development in mice on a standard diet. Public Library of Science 2018 adipocyte sphingolipid synthesis diet-dependent sphingolipid synthesis diet-induced adipose tissue expansion adipocyte lipid metabolism lipid phosphatase activity LPP 3 LPP 3 substrate sphingosine 1- phosphate serine palmitoyl transferase intracellular PA-specific phosphatases specificity membrane-associated lipid phosphate phosphatases LPP 3 deficiency Adipocyte LPP 3 deficiency lipin 1 causes lipodystrophy Lipid phosphate phosphatase 3 adipocyte LPP 3 deficiency Western diet-fed mice 2018-06-11 17:31:24 Figure https://plos.figshare.com/articles/figure/Deficiency_of_adipose_LPP3_does_not_impact_body_size_or_adipose_tissue_development_in_mice_on_a_standard_diet_/6482048 <p>Body weight of male (A) and female (B) <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open symbols) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed symbols) mice. Results are graphed as mean ± SD from 4–5 animals/group. Weight in grams of interscapular brown adipose tissue (IBAT), subcutaneous (SUB) white adipose tissue, and liver, and femur length (in mm) in 9-week old male (C) and female (D) <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open bars) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed bars) mice. Comparisons between genotypes were made by Students t-test. E. Representative histology of <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ) subcutaneous white adipose tissue stained with Periodic acid–Schiff (PAS) at 10x (left) and 20x (right) magnification. F. Expression analysis of inflammatory gene expression in visceral fat from <i>Plpp3</i><sup><i>fl/fl</i></sup> (fl/fl; open bars; n = 5) and AP2-Cre/<i>Plpp3</i><sup>Δ</sup> (Δ; closed bars; n = 4) mice on high fat diet. Results are graphed as mean ± SD and data analyzed by two-tailed t-test.</p>