%0 Figure %A Takahashi, Makoto %A Obayashi, Masato %A Ishiguro, Taro %A Sato, Nozomu %A Niimi, Yusuke %A Ozaki, Kokoro %A Mogushi, Kaoru %A Mahmut, Yasen %A Tanaka, Hiroshi %A Tsuruta, Fuminori %A Dolmetsch, Ricardo %A Yamada, Mitsunori %A Takahashi, Hitoshi %A Kato, Takeo %A Mori, Osamu %A Eishi, Yoshinobu %A Mizusawa, Hidehiro %A Ishikawa, Kinya %D 2013 %T CREB co-localizes with intracytoplasmic CTF aggregates and quantity of CREB is decreased in cultured cell model. %U https://plos.figshare.com/articles/figure/_CREB_co_localizes_with_intracytoplasmic_CTF_aggregates_and_quantity_of_CREB_is_decreased_in_cultured_cell_model_/647024 %R 10.1371/journal.pone.0050121.g004 %2 https://plos.figshare.com/ndownloader/files/980747 %K co-localizes %K intracytoplasmic %K ctf %K aggregates %K creb %K cultured %X

(A) In non-transfected PC12 cells, CREB and p-CREB-immunofluorescence labeling is strong in the nucleus, while the cytoplasm shows only weak and diffuse immunofluorescence. (B) In PC12 cells over-expressing rCTF-Q28-NES, the cytoplasmic CTF aggregates co-localize with CREB (upper row) and p-CREB (lower row), showing focally strong immunofluorescence in their cell bodies (arrows). (C&D) In contrast to the Dox(+) stable PC12 cells (upper row in C&D), co-localizations of CREB (lower row in C) or p-CREB (lower row in D) with cytoplasmic aggregates are also observed in Dox(−) stable PC12 cells expressing rCTF-Q28-NES (arrows). (E) The quantities of CREB and p-CREB in the nucleus were both decreased when the rCTF was expressed in the cytoplasm, but not so when the rCTF was targeted in the nucleus. (For A-D: scale bars: 10 µm).

%I PLOS ONE