10.1371/journal.pone.0198613.g004 Lisa Nonaka Lisa Nonaka Tatsuya Yamamoto Tatsuya Yamamoto Fumito Maruyama Fumito Maruyama Yuu Hirose Yuu Hirose Yuki Onishi Yuki Onishi Takeshi Kobayashi Takeshi Kobayashi Satoru Suzuki Satoru Suzuki Nobuhiko Nomura Nobuhiko Nomura Michiaki Masuda Michiaki Masuda Hirokazu Yano Hirokazu Yano Excision of Tn<i>6283</i> from the <i>E</i>. <i>coli</i> chromosome. Public Library of Science 2018 Tn 6283 copy element Tn 6283 Vibrio ponticus interspecies gene transfer plasmid transfer gene transfer model Molecular genetics analysis MGE interspecies ARGs transfer self-transmissible plasmid backbone encode transfer function MOB H group chromosome antimicrobial resistance genes pSEA 1. Tn 6283 2018-06-07 17:46:40 Figure https://plos.figshare.com/articles/figure/Excision_of_Tn_i_6283_i_from_the_i_E_i_i_coli_i_chromosome_/6458129 <p>(A) Design for PCR detection of recombination products. The diagrams show the hypothetical scenario in which Tn<i>6283</i> excises itself as a circular molecule and forms a heteroduplex joint, while the Tn<i>6283</i> donor site also forms a heteroduplex joint. The PCR-amplified heteroduplex joints should contain two types of sequences in the spacer between terminal repeat sequences. (B) PCR detection of joint formation on the recombination products. Lane 1: long PCR designed to detect the occupied Tn<i>6283</i> donor site (primer set 2599572R-2599370F). Lane 2: detection of unoccupied Tn<i>6283</i> donor sites (primer set 2599572R-2599370F). Lane 3: detection of circularized Tn<i>6283</i> (primer set 3F-3R). (C) Sequences of PCR-amplified joints on the circularized Tn<i>6283</i>. The observed frequency is indicated next to each sequence. (D) Sequences of PCR-amplified joints on the unoccupied Tn<i>6283</i> donor sites. In the upper panel, two types of expected joint sequences are shown. The lower panel shows the unexpectedly observed sequence, designated Scar.</p>