10.1371/journal.pone.0198613.g004
Lisa Nonaka
Lisa
Nonaka
Tatsuya Yamamoto
Tatsuya
Yamamoto
Fumito Maruyama
Fumito
Maruyama
Yuu Hirose
Yuu
Hirose
Yuki Onishi
Yuki
Onishi
Takeshi Kobayashi
Takeshi
Kobayashi
Satoru Suzuki
Satoru
Suzuki
Nobuhiko Nomura
Nobuhiko
Nomura
Michiaki Masuda
Michiaki
Masuda
Hirokazu Yano
Hirokazu
Yano
Excision of Tn<i>6283</i> from the <i>E</i>. <i>coli</i> chromosome.
Public Library of Science
2018
Tn 6283 copy
element Tn 6283
Vibrio ponticus
interspecies gene transfer
plasmid transfer
gene transfer model
Molecular genetics analysis
MGE
interspecies ARGs transfer
self-transmissible plasmid backbone
encode transfer function
MOB H group
chromosome
antimicrobial resistance genes
pSEA 1. Tn 6283
2018-06-07 17:46:40
Figure
https://plos.figshare.com/articles/figure/Excision_of_Tn_i_6283_i_from_the_i_E_i_i_coli_i_chromosome_/6458129
<p>(A) Design for PCR detection of recombination products. The diagrams show the hypothetical scenario in which Tn<i>6283</i> excises itself as a circular molecule and forms a heteroduplex joint, while the Tn<i>6283</i> donor site also forms a heteroduplex joint. The PCR-amplified heteroduplex joints should contain two types of sequences in the spacer between terminal repeat sequences. (B) PCR detection of joint formation on the recombination products. Lane 1: long PCR designed to detect the occupied Tn<i>6283</i> donor site (primer set 2599572R-2599370F). Lane 2: detection of unoccupied Tn<i>6283</i> donor sites (primer set 2599572R-2599370F). Lane 3: detection of circularized Tn<i>6283</i> (primer set 3F-3R). (C) Sequences of PCR-amplified joints on the circularized Tn<i>6283</i>. The observed frequency is indicated next to each sequence. (D) Sequences of PCR-amplified joints on the unoccupied Tn<i>6283</i> donor sites. In the upper panel, two types of expected joint sequences are shown. The lower panel shows the unexpectedly observed sequence, designated Scar.</p>