Chen, Zheyan Lee, Han J. Henle, Steven R. Cheever, Thomas C. Ekker, Stephen R. Henley, John BDNF stimulated outgrowth is Ca<sup>2+</sup> dependent and requires Ca<sup>2+</sup> release from internal stores. <p>(A) Bath application of BDNF elicits a strong Ca<sup>2+</sup> signal in zebrafish growth cones (pseudocolored according to scale on left, with blue  =  lower [Ca<sup>2+</sup>] and white  =  higher [Ca<sup>2+</sup>]), quantified in (B). Treatment with both (C) BAPTA-AM, a cytoplasmic Ca<sup>2+</sup> buffer, and (D) thapsigargin, which depletes intracellular Ca<sup>2+</sup> pools, abolish BDNF stimulated outgrowth. Scale bar, 5 µm. *, p<0.05; ns, not significant.</p> stimulated;outgrowth;requires 2013-03-05
    https://plos.figshare.com/articles/figure/_BDNF_stimulated_outgrowth_is_Ca_2_dependent_and_requires_Ca_2_release_from_internal_stores_/644120
10.1371/journal.pone.0057539.g006