Effect of ox-ApoE on triglyceride, cholesterol and PC efflux from foam cells. Youfeng Yang Zehong Cao Ling Tian W. Timothy Garvey Guangjie Cheng 10.1371/journal.pone.0057571.g005 https://plos.figshare.com/articles/figure/_Effect_of_ox_ApoE_on_triglyceride_cholesterol_and_PC_efflux_from_foam_cells_/640016 <p>A. Cellular triglyceride efflux by native and ox-ApoE. B. Cellular cholesterol efflux by native and ox-ApoE. C. Cellular PC efflux by native and ox-ApoE. D. Detection of triglycerides in supernatant. E. Detection of cholesterol in supernatant. F. Detection of PC in supernatant. THP-1 cells were loaded with lipids as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057571#s2" target="_blank">Materials and Methods</a>. Efflux of triglycerides, cholesterol, and PC was initiated by addition of native or ox-ApoE in cultured foam cells. After treatment of 9 hrs, triglycerides, cholesterol, and PC in supernatant were measured by the respect kit. After treatment of 24 hrs, the cellular triglycerides, cholesterol, and PC were measured with the respect kit. Tg: triglycerides; Ch: cholesterol; PC: phosphatidylcholine. Data are shown as means ± SEM; *p<0.05 vs. PBS group. n = 3.</p> 2013-02-26 12:08:10 ox-apoe pc efflux foam