%0 Figure %A Yang, Youfeng %A Cao, Zehong %A Tian, Ling %A Timothy Garvey, W. %A Cheng, Guangjie %D 2013 %T Ox-ApoE binds weakly to emulsion particles. %U https://plos.figshare.com/articles/figure/_Ox_ApoE_binds_weakly_to_emulsion_particles_/640014 %R 10.1371/journal.pone.0057571.g004 %2 https://plos.figshare.com/ndownloader/files/970875 %K binds %K weakly %K emulsion %X

A. Ox-ApoE binding with triglyceride-PC emulsion particles. Native and ox-ApoE were incubated with triglyceride-PC emulsion particles at room temperature for 1 hr with gentle shaking. Emulsion particles and lipid-free buffer were segregated by centrifugation. Equal amount of samples from top and lower layers (contain lipid-bound ApoE and free ApoE, respectively) were carried out for Western blot analysis using anti-ApoE antibody. B. Ox-ApoE binding with PC emulsion particles. Native and ox-ApoE were incubated with PC emulsion particles at room temperature for 1 hr with gentle shaking. Emulsion particles and lipid-free buffer were segregated by centrifugation as in A. Equal amount of samples from top and lower layers (contain lipid-bound ApoE and free ApoE, respectively) were carried out for Western blot analysis using anti-ApoE antibody. C. Quantification of ApoE and ox-ApoE binding to triglycerides-PC emulsion particles from data in A. The ratio was calculated as emulsion particle-bound ApoE/free ApoE. D. Quantification of ApoE and ox-ApoE binding to PC emulsion particles from data in B. The ratio was calculated as emulsion particle-bound ApoE/free ApoE. Data are shown as means ± SEM; *p<0.05. n = 3.

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