1,25(OH)<sub>2</sub>D<sub>3</sub> administration regulated the protein abundance of NaPi-IIa, klotho and Vdr. Larissa Kägi Carla Bettoni Eva M. Pastor-Arroyo Udo Schnitzbauer Nati Hernando Carsten A. Wagner 10.1371/journal.pone.0195427.g009 https://plos.figshare.com/articles/figure/1_25_OH_sub_2_sub_D_sub_3_sub_administration_regulated_the_protein_abundance_of_NaPi-IIa_klotho_and_Vdr_/6283676 <p>Representative western blots of total renal protein homogenates from mice injected with 1,25(OH)<sub>2</sub>D<sub>3</sub> either once within 14 hours (14h) or twice within 96 hours (96h) and the corresponding controls using antibodies against NaPi-IIa <b>(A)</b>, full length Klotho <b>(B)</b>, Cyp24a1 <b>(C)</b> and Vdr <b>(D)</b>. The proteins of interest are shown in the top panels, whereas the corresponding β-actin signal is shown in the bottom image. Densitometric quantification was normalized to the corresponding β-actin values. Bar graphs represent mean ± SD (n = 6/group) of the normalized values and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.</p> 2018-05-17 08:17:15 2 D 3 fibroblast growth factor 23 vitamin D metabolizing enzymes 2 D 3 synthesis OH Pi 2 D 3 Background extrarenal tissues Cyp 24a 25- hydroxylases CYP 2R vitamin D hydroxylases 27A 27B expression 2 D 3 treatment 24- hydroxylase CYP 24A catalyzes vitamin D receptor VDR cytochrome P 450 FGF 23 PTH