1,25(OH)<sub>2</sub>D<sub>3</sub> treatment mainly changed renal mRNA expression. Larissa Kägi Carla Bettoni Eva M. Pastor-Arroyo Udo Schnitzbauer Nati Hernando Carsten A. Wagner 10.1371/journal.pone.0195427.g008 https://plos.figshare.com/articles/figure/1_25_OH_sub_2_sub_D_sub_3_sub_treatment_mainly_changed_renal_mRNA_expression_/6283673 <p>mRNA expression of <i>Cyp27a1</i> <b>(A)</b>, <i>Cyp2r1</i> <b>(B)</b>, <i>Cyp27b1</i> <b>(C)</b>, <i>Cyp24a1</i> <b>(D)</b> and <i>Vdr</i> <b>(E)</b> were measured in kidney (Ki), liver (Li), small intestine (Si), colon (Co), bone (Bo), abdominal fat (Af) and brain (Br) samples of mice injected with 1,25(OH)<sub>2</sub>D<sub>3</sub> either once within 14 hours (14h, grey bars) or twice within 96 hours (96h, black bars) and their corresponding controls (white and striped bars, respectively). Expression of the mRNAs of interest was normalized to <i>Gapdh</i> (<i>Hprt</i> was used for normalization in bone). Graphs represent mean ± SD (n = 6/group) of relative changes (given as percentage and normalized to controls at 14 and 96 hrs, respectively) and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.</p> 2018-05-17 08:17:15 2 D 3 fibroblast growth factor 23 vitamin D metabolizing enzymes 2 D 3 synthesis OH Pi 2 D 3 Background extrarenal tissues Cyp 24a 25- hydroxylases CYP 2R vitamin D hydroxylases 27A 27B expression 2 D 3 treatment 24- hydroxylase CYP 24A catalyzes vitamin D receptor VDR cytochrome P 450 FGF 23 PTH