10.1371/journal.pone.0195427.g008
Larissa Kägi
Larissa
Kägi
Carla Bettoni
Carla
Bettoni
Eva M. Pastor-Arroyo
Eva
M. Pastor-Arroyo
Udo Schnitzbauer
Udo
Schnitzbauer
Nati Hernando
Nati
Hernando
Carsten A. Wagner
Carsten
A. Wagner
1,25(OH)<sub>2</sub>D<sub>3</sub> treatment mainly changed renal mRNA expression.
Public Library of Science
2018
2 D 3
fibroblast growth factor 23
vitamin D metabolizing enzymes
2 D 3 synthesis
OH
Pi
2 D 3 Background
extrarenal tissues
Cyp 24a
25- hydroxylases CYP 2R
vitamin D hydroxylases
27A
27B
expression
2 D 3 treatment
24- hydroxylase CYP 24A catalyzes
vitamin D receptor VDR
cytochrome P 450
FGF 23
PTH
2018-05-17 08:17:15
Figure
https://plos.figshare.com/articles/figure/1_25_OH_sub_2_sub_D_sub_3_sub_treatment_mainly_changed_renal_mRNA_expression_/6283673
<p>mRNA expression of <i>Cyp27a1</i> <b>(A)</b>, <i>Cyp2r1</i> <b>(B)</b>, <i>Cyp27b1</i> <b>(C)</b>, <i>Cyp24a1</i> <b>(D)</b> and <i>Vdr</i> <b>(E)</b> were measured in kidney (Ki), liver (Li), small intestine (Si), colon (Co), bone (Bo), abdominal fat (Af) and brain (Br) samples of mice injected with 1,25(OH)<sub>2</sub>D<sub>3</sub> either once within 14 hours (14h, grey bars) or twice within 96 hours (96h, black bars) and their corresponding controls (white and striped bars, respectively). Expression of the mRNAs of interest was normalized to <i>Gapdh</i> (<i>Hprt</i> was used for normalization in bone). Graphs represent mean ± SD (n = 6/group) of relative changes (given as percentage and normalized to controls at 14 and 96 hrs, respectively) and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.</p>