10.1371/journal.pone.0195427.g008 Larissa Kägi Larissa Kägi Carla Bettoni Carla Bettoni Eva M. Pastor-Arroyo Eva M. Pastor-Arroyo Udo Schnitzbauer Udo Schnitzbauer Nati Hernando Nati Hernando Carsten A. Wagner Carsten A. Wagner 1,25(OH)<sub>2</sub>D<sub>3</sub> treatment mainly changed renal mRNA expression. Public Library of Science 2018 2 D 3 fibroblast growth factor 23 vitamin D metabolizing enzymes 2 D 3 synthesis OH Pi 2 D 3 Background extrarenal tissues Cyp 24a 25- hydroxylases CYP 2R vitamin D hydroxylases 27A 27B expression 2 D 3 treatment 24- hydroxylase CYP 24A catalyzes vitamin D receptor VDR cytochrome P 450 FGF 23 PTH 2018-05-17 08:17:15 Figure https://plos.figshare.com/articles/figure/1_25_OH_sub_2_sub_D_sub_3_sub_treatment_mainly_changed_renal_mRNA_expression_/6283673 <p>mRNA expression of <i>Cyp27a1</i> <b>(A)</b>, <i>Cyp2r1</i> <b>(B)</b>, <i>Cyp27b1</i> <b>(C)</b>, <i>Cyp24a1</i> <b>(D)</b> and <i>Vdr</i> <b>(E)</b> were measured in kidney (Ki), liver (Li), small intestine (Si), colon (Co), bone (Bo), abdominal fat (Af) and brain (Br) samples of mice injected with 1,25(OH)<sub>2</sub>D<sub>3</sub> either once within 14 hours (14h, grey bars) or twice within 96 hours (96h, black bars) and their corresponding controls (white and striped bars, respectively). Expression of the mRNAs of interest was normalized to <i>Gapdh</i> (<i>Hprt</i> was used for normalization in bone). Graphs represent mean ± SD (n = 6/group) of relative changes (given as percentage and normalized to controls at 14 and 96 hrs, respectively) and data was analyzed by unpaired student’s t-test with significant p values indicated as: * p ≤ 0.05, ** p ≤ 0.01 and *** p < 0.0001.</p>