Western Blot Analysis of ITPR1 Protein Levels in EBV Immortalized Lymphoblasts from AUS1 Family Members
Joyce van de Leemput
Jayanth Chandran
Melanie A Knight
Lynne A Holtzclaw
Sonja Scholz
Mark R Cookson
Henry Houlden
Katrina Gwinn-Hardy
Hon-Chung Fung
Xian Lin
Dena Hernandez
Javier Simon-Sanchez
Nick W Wood
Paola Giunti
Ian Rafferty
John Hardy
Elsdon Storey
R. J. McKinlay Gardner
Susan M Forrest
Elizabeth M. C Fisher
James T Russell
Huaibin Cai
Andrew B Singleton
10.1371/journal.pgen.0030108.g004
https://plos.figshare.com/articles/figure/_Western_Blot_Analysis_of_ITPR1_Protein_Levels_in_EBV_Immortalized_Lymphoblasts_from_AUS1_Family_Members_/619436
<p>Western blot performed to examine ITPR1 levels in EBV immortalized lymphocytes from AUS1 affected family members carrying the <i>ITPR1</i> deletion and from an AUS1 family member of unknown disease status who does not carry the deletion. Notably the samples from patients with <i>ITPR1</i> deletion show a dramatic decrease in ITPR1 levels. To demonstrate equal loading, these samples were diluted one in five, and the Western blot was repeated using an antibody against ACTB.</p>
2007-06-22 02:37:16
blot
itpr1
ebv
immortalized
lymphoblasts
aus1