Western Blot Analysis of ITPR1 Protein Levels in EBV Immortalized Lymphoblasts from AUS1 Family Members Joyce van de Leemput Jayanth Chandran Melanie A Knight Lynne A Holtzclaw Sonja Scholz Mark R Cookson Henry Houlden Katrina Gwinn-Hardy Hon-Chung Fung Xian Lin Dena Hernandez Javier Simon-Sanchez Nick W Wood Paola Giunti Ian Rafferty John Hardy Elsdon Storey R. J. McKinlay Gardner Susan M Forrest Elizabeth M. C Fisher James T Russell Huaibin Cai Andrew B Singleton 10.1371/journal.pgen.0030108.g004 https://plos.figshare.com/articles/figure/_Western_Blot_Analysis_of_ITPR1_Protein_Levels_in_EBV_Immortalized_Lymphoblasts_from_AUS1_Family_Members_/619436 <p>Western blot performed to examine ITPR1 levels in EBV immortalized lymphocytes from AUS1 affected family members carrying the <i>ITPR1</i> deletion and from an AUS1 family member of unknown disease status who does not carry the deletion. Notably the samples from patients with <i>ITPR1</i> deletion show a dramatic decrease in ITPR1 levels. To demonstrate equal loading, these samples were diluted one in five, and the Western blot was repeated using an antibody against ACTB.</p> 2007-06-22 02:37:16 blot itpr1 ebv immortalized lymphoblasts aus1