ELSD chromatograms for the methanolic extract before and after enrichment, and its fractionation by preparative chromatography. Théo Brillatz Chiara Lauritano Maxime Jacmin Supitcha Khamma Laurence Marcourt Davide Righi Giovanna Romano Francesco Esposito Adrianna Ianora Emerson F. Queiroz Jean-Luc Wolfender Alexander D. Crawford 10.1371/journal.pone.0196195.g002 https://plos.figshare.com/articles/figure/ELSD_chromatograms_for_the_methanolic_extract_before_and_after_enrichment_and_its_fractionation_by_preparative_chromatography_/6178784 <p>(A) UHPLC chromatogram of the crude methanolic extract exhibiting mainly unretained polar metabolites. (B) UHPLC metabolite profiling after enrichment by VLC to remove very polar residues. (C) Preparative chromatography for the fractionation of the enriched extract highlighting the isolation of constituent <b>1</b>.</p> 2018-04-24 17:41:03 bioassay-guided fractionation diatom Skeletonema marinoi marine diatom Skeletonema marinoi anticonvulsant activity UHPLC-HRMS adenosine receptor agonist microalgae neuroactive antiseizure nucleoside inosine vivo zebrafish bioassay constituent zebrafish epilepsy model metabolite rat epilepsy models GABA