10.1371/journal.pone.0196195.g002 Théo Brillatz Théo Brillatz Chiara Lauritano Chiara Lauritano Maxime Jacmin Maxime Jacmin Supitcha Khamma Supitcha Khamma Laurence Marcourt Laurence Marcourt Davide Righi Davide Righi Giovanna Romano Giovanna Romano Francesco Esposito Francesco Esposito Adrianna Ianora Adrianna Ianora Emerson F. Queiroz Emerson F. Queiroz Jean-Luc Wolfender Jean-Luc Wolfender Alexander D. Crawford Alexander D. Crawford ELSD chromatograms for the methanolic extract before and after enrichment, and its fractionation by preparative chromatography. Public Library of Science 2018 bioassay-guided fractionation diatom Skeletonema marinoi marine diatom Skeletonema marinoi anticonvulsant activity UHPLC-HRMS adenosine receptor agonist microalgae neuroactive antiseizure nucleoside inosine vivo zebrafish bioassay constituent zebrafish epilepsy model metabolite rat epilepsy models GABA 2018-04-24 17:41:03 Figure https://plos.figshare.com/articles/figure/ELSD_chromatograms_for_the_methanolic_extract_before_and_after_enrichment_and_its_fractionation_by_preparative_chromatography_/6178784 <p>(A) UHPLC chromatogram of the crude methanolic extract exhibiting mainly unretained polar metabolites. (B) UHPLC metabolite profiling after enrichment by VLC to remove very polar residues. (C) Preparative chromatography for the fractionation of the enriched extract highlighting the isolation of constituent <b>1</b>.</p>