FAC-treated murine alveolar macrophage cells, MH-S. Jungnam Lee Ivan Arisi Ermanno Puxeddu Lazarus K. Mramba Massimo Amicosante Carmen M. Swaisgood Marco Pallante Mark L. Brantly C. Magnus Sköld Cesare Saltini 10.1371/journal.pone.0194803.g005 https://plos.figshare.com/articles/figure/FAC-treated_murine_alveolar_macrophage_cells_MH-S_/6134753 <p>Murine alveolar macrophage cells (MH-S, ATCC CRL-2019) derived from a BALB/C strain mouse were incubated with 100 μM of Ferric ammonium citrate (FAC) for 1, 3, and 5 days. <i>A</i>. Shown are iron and ROS levels in FAC-treated cells. <i>B</i>. Shown are relative mRNA expression levels of selected chemokines/cytokines in FAC-treated mouse alveolar macrophages examined by qRT-PCR. Relative expression levels of the cytokines were normalized to the GNB2L1 housekeeping gene and calculated using the 2^ΔΔCt method. Mann-Whitney test (*) and (**) denote statistical significance (P < 0.05, P < 0.01, respectively) vs. control.</p> 2018-04-12 17:36:44 p-value angiogenic activation pattern macrophage activation fibrosis BAL cells Gene expression analysis Cxcl RNA alveolar epithelial damage CTR macrophage iron accumulation Idiopathic KEGG ferric ammonium citrate iron-dependent ROS generation data show alveolar macrophage expression MH-S alveolar macrophage activation phenotype upregulated M 1-type MTPP IPF patients