FAC-treated murine alveolar macrophage cells, MH-S.
Jungnam Lee
Ivan Arisi
Ermanno Puxeddu
Lazarus K. Mramba
Massimo Amicosante
Carmen M. Swaisgood
Marco Pallante
Mark L. Brantly
C. Magnus Sköld
Cesare Saltini
10.1371/journal.pone.0194803.g005
https://plos.figshare.com/articles/figure/FAC-treated_murine_alveolar_macrophage_cells_MH-S_/6134753
<p>Murine alveolar macrophage cells (MH-S, ATCC CRL-2019) derived from a BALB/C strain mouse were incubated with 100 μM of Ferric ammonium citrate (FAC) for 1, 3, and 5 days. <i>A</i>. Shown are iron and ROS levels in FAC-treated cells. <i>B</i>. Shown are relative mRNA expression levels of selected chemokines/cytokines in FAC-treated mouse alveolar macrophages examined by qRT-PCR. Relative expression levels of the cytokines were normalized to the GNB2L1 housekeeping gene and calculated using the 2^ΔΔCt method. Mann-Whitney test (*) and (**) denote statistical significance (P < 0.05, P < 0.01, respectively) vs. control.</p>
2018-04-12 17:36:44
p-value
angiogenic activation pattern
macrophage activation
fibrosis
BAL cells
Gene expression analysis
Cxcl
RNA
alveolar epithelial damage
CTR
macrophage iron accumulation Idiopathic
KEGG
ferric ammonium citrate
iron-dependent ROS generation
data show alveolar macrophage expression
MH-S
alveolar macrophage activation phenotype
upregulated M 1-type
MTPP
IPF patients