10.1371/journal.pone.0194803.g005 Jungnam Lee Jungnam Lee Ivan Arisi Ivan Arisi Ermanno Puxeddu Ermanno Puxeddu Lazarus K. Mramba Lazarus K. Mramba Massimo Amicosante Massimo Amicosante Carmen M. Swaisgood Carmen M. Swaisgood Marco Pallante Marco Pallante Mark L. Brantly Mark L. Brantly C. Magnus Sköld C. Magnus Sköld Cesare Saltini Cesare Saltini FAC-treated murine alveolar macrophage cells, MH-S. Public Library of Science 2018 p-value angiogenic activation pattern macrophage activation fibrosis BAL cells Gene expression analysis Cxcl RNA alveolar epithelial damage CTR macrophage iron accumulation Idiopathic KEGG ferric ammonium citrate iron-dependent ROS generation data show alveolar macrophage expression MH-S alveolar macrophage activation phenotype upregulated M 1-type MTPP IPF patients 2018-04-12 17:36:44 Figure https://plos.figshare.com/articles/figure/FAC-treated_murine_alveolar_macrophage_cells_MH-S_/6134753 <p>Murine alveolar macrophage cells (MH-S, ATCC CRL-2019) derived from a BALB/C strain mouse were incubated with 100 μM of Ferric ammonium citrate (FAC) for 1, 3, and 5 days. <i>A</i>. Shown are iron and ROS levels in FAC-treated cells. <i>B</i>. Shown are relative mRNA expression levels of selected chemokines/cytokines in FAC-treated mouse alveolar macrophages examined by qRT-PCR. Relative expression levels of the cytokines were normalized to the GNB2L1 housekeeping gene and calculated using the 2^ΔΔCt method. Mann-Whitney test (*) and (**) denote statistical significance (P < 0.05, P < 0.01, respectively) vs. control.</p>