10.1371/journal.pone.0194803.g005
Jungnam Lee
Jungnam
Lee
Ivan Arisi
Ivan
Arisi
Ermanno Puxeddu
Ermanno
Puxeddu
Lazarus K. Mramba
Lazarus K.
Mramba
Massimo Amicosante
Massimo
Amicosante
Carmen M. Swaisgood
Carmen M.
Swaisgood
Marco Pallante
Marco
Pallante
Mark L. Brantly
Mark
L. Brantly
C. Magnus Sköld
C.
Magnus Sköld
Cesare Saltini
Cesare
Saltini
FAC-treated murine alveolar macrophage cells, MH-S.
Public Library of Science
2018
p-value
angiogenic activation pattern
macrophage activation
fibrosis
BAL cells
Gene expression analysis
Cxcl
RNA
alveolar epithelial damage
CTR
macrophage iron accumulation Idiopathic
KEGG
ferric ammonium citrate
iron-dependent ROS generation
data show alveolar macrophage expression
MH-S
alveolar macrophage activation phenotype
upregulated M 1-type
MTPP
IPF patients
2018-04-12 17:36:44
Figure
https://plos.figshare.com/articles/figure/FAC-treated_murine_alveolar_macrophage_cells_MH-S_/6134753
<p>Murine alveolar macrophage cells (MH-S, ATCC CRL-2019) derived from a BALB/C strain mouse were incubated with 100 μM of Ferric ammonium citrate (FAC) for 1, 3, and 5 days. <i>A</i>. Shown are iron and ROS levels in FAC-treated cells. <i>B</i>. Shown are relative mRNA expression levels of selected chemokines/cytokines in FAC-treated mouse alveolar macrophages examined by qRT-PCR. Relative expression levels of the cytokines were normalized to the GNB2L1 housekeeping gene and calculated using the 2^ΔΔCt method. Mann-Whitney test (*) and (**) denote statistical significance (P < 0.05, P < 0.01, respectively) vs. control.</p>