10.1371/journal.pone.0001985.g002 Wei Li Wei Li Yan Liu Yan Liu Muhammad Mahmood Mukhtar Muhammad Mahmood Mukhtar Rui Gong Rui Gong Ying Pan Ying Pan Sahibzada T. Rasool Sahibzada T. Rasool Yecheng Gao Yecheng Gao Lei Kang Lei Kang Qian Hao Qian Hao Guiqing Peng Guiqing Peng Yanni Chen Yanni Chen Xin Chen Xin Chen Jianguo Wu Jianguo Wu Ying Zhu Ying Zhu Screening of viral proteins or viral replicative intermediate dsRNA involved in induction of COX-2/ IL-32. Public Library of Science 2013 viral proteins replicative intermediate dsrna induction 2013-02-21 10:37:17 Figure https://plos.figshare.com/articles/figure/_Screening_of_viral_proteins_or_viral_replicative_intermediate_dsRNA_involved_in_induction_of_COX_2_IL_32_/603501 <p>Reporter plasmids pCOX-2-Luc (A), pIL-32-Luc (B), and pRL-TK were cotransfected along with all 10 viral gene constructs (Flag2A-HA, Flag2A-NA, Flag2A-NP, Flag2A-NS1, Flag2A-NS2, Flag2A-M1, Flag2A-M2, Flag2A-PA, Flag2A-PB1, Flag2A-PB2) and control vectors (Flag2A) or treated with or without poly(IC) (50 µg/ml), poly(IC) (50 µg/ml)+IFN-γ (150 U/ml) into A549 cells which are indicated on the horizontal axis respectively. Luciferase activity was measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001985#s2" target="_blank">methods</a> section. Results are expressed as the mean±s.d. of three independent experiments performed in triplicate and normalized by Renilla activities.</p>