10.1371/journal.pone.0001985.g002
Wei Li
Wei
Li
Yan Liu
Yan
Liu
Muhammad Mahmood Mukhtar
Muhammad
Mahmood Mukhtar
Rui Gong
Rui
Gong
Ying Pan
Ying
Pan
Sahibzada T. Rasool
Sahibzada
T. Rasool
Yecheng Gao
Yecheng
Gao
Lei Kang
Lei
Kang
Qian Hao
Qian
Hao
Guiqing Peng
Guiqing
Peng
Yanni Chen
Yanni
Chen
Xin Chen
Xin
Chen
Jianguo Wu
Jianguo
Wu
Ying Zhu
Ying
Zhu
Screening of viral proteins or viral replicative intermediate dsRNA involved in induction of COX-2/ IL-32.
Public Library of Science
2013
viral
proteins
replicative
intermediate
dsrna
induction
2013-02-21 10:37:17
Figure
https://plos.figshare.com/articles/figure/_Screening_of_viral_proteins_or_viral_replicative_intermediate_dsRNA_involved_in_induction_of_COX_2_IL_32_/603501
<p>Reporter plasmids pCOX-2-Luc (A), pIL-32-Luc (B), and pRL-TK were cotransfected along with all 10 viral gene constructs (Flag2A-HA, Flag2A-NA, Flag2A-NP, Flag2A-NS1, Flag2A-NS2, Flag2A-M1, Flag2A-M2, Flag2A-PA, Flag2A-PB1, Flag2A-PB2) and control vectors (Flag2A) or treated with or without poly(IC) (50 µg/ml), poly(IC) (50 µg/ml)+IFN-γ (150 U/ml) into A549 cells which are indicated on the horizontal axis respectively. Luciferase activity was measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001985#s2" target="_blank">methods</a> section. Results are expressed as the mean±s.d. of three independent experiments performed in triplicate and normalized by Renilla activities.</p>