Reduced CD8<sup>+</sup>T lymphocytes infiltration in the brain of PbA-infected LTβR deficient mice. TogbeDieudonnée Loureiro de SousaPaulo FauconnierMathilde BoissayVictorine FickLizette ScheuStefanie PfefferKlaus MenardRobert GrauGeorges E. DoanBich-Thuy Claude BeloeilJean ReniaLaurent M. HansenAnna J. BallHelen HuntNicholas H. RyffelBernhard F. J. QuesniauxValerie 2008 <p>Flow cytometric analyses of brain-sequestered leukocytes from uninfected wild-type mice (WT NI), PbA-infected wild-type mice (WT PbA) and TNF deficient mice with severe ECM, and infected, ECM resistant LTβ and LTβR deficient mice tested on the same day. Mice were infected with 10<sup>6 </sup><i>P. berghei ANKA</i> parasitized red blood cells as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002608#pone-0002608-g001" target="_blank">Fig. 1</a>. Representative dot blots of CD4 and CD8 staining (A) CD8 and perforin double staining (B). Activation markers CD54 (C) and CD69 (D) on CD8<sup>+</sup> cells are shown for non infected (thin line) and PbA infected (thick line) WT, TNF-, LTβR- and LTβ–deficient mice, as indicated. Bargraphs of CD4<sup>+</sup> (E), CD8<sup>+</sup> (F), CD8<sup>+</sup> Perforin<sup>+</sup> (G), CD8<sup>+</sup> CD54<sup>+</sup> (H), and CD8<sup>+</sup> CD69<sup>+</sup> (I) lymphocytes represent the mean±SD of n = 3–4 pools of 3–4 mice per group, and are from one representative of two independent experiments (n.s., not significant).</p>