Merozoite invasion properties of <i>P. falciparum</i> W2mef, FCR3, Dd2, 3D7 and HB3 clones. Xiaohong Gao Kim Pin Yeo Siqi Sharon Aw Claudia Kuss Jayasree K. Iyer Saraswathy Genesan Ravikumar Rajamanonmani Julien Lescar Zbynek Bozdech Peter R. Preiser 10.1371/journal.ppat.1000104.g005 https://plos.figshare.com/articles/figure/_Merozoite_invasion_properties_of_P_falciparum_W2mef_FCR3_Dd2_3D7_and_HB3_clones_/597697 <p>(A) Comparison of merozoite invasion inhibition with αrRII-3 and αrtRVIII, including pre-immune serum in 1∶10 and 1∶640 dilutions. Positive control was the invasion of merozoites into the normal complete medium. The error bar denotes the SE. * <i>p</i><0.001, indicating that the invasion of W2mef, FCR3 and Dd2 were successfully inhibited by αrRII-3, compared to 3D7 and HB3. + <i>p</i><0.05 indicating that Dd2 exhibit an intermediate sensitivity to inhibitory antiserum as compared to W2mef and FCR3. (B) Parasites were also assayed for their ability to invade neuraminidase-treated erythrocytes (1mU/ml final). For each parasite clone, the percentage of inhibition of the ability to invade neuraminidase-treated erythrocytes (vertical axis) is plotted against the percentage of invasion inhibition of αrRII-3 in a 1∶10 dilution (horizonal axis). Western blot of enriched culture supernatant from all 5 clones used in this study and probed with αrRII-3, αrtRVIII and αSERA5 was shown in the insert of (B). No RH1 protein was detected in 3D7 and HB3 clones compared with W2mef, Dd2 and FCR3.</p> 2008-07-11 02:08:17 3d7 hb3