10.1371/journal.ppat.1000104.g005 Xiaohong Gao Xiaohong Gao Kim Pin Yeo Kim Pin Yeo Siqi Sharon Aw Siqi Sharon Aw Claudia Kuss Claudia Kuss Jayasree K. Iyer Jayasree K. Iyer Saraswathy Genesan Saraswathy Genesan Ravikumar Rajamanonmani Ravikumar Rajamanonmani Julien Lescar Julien Lescar Zbynek Bozdech Zbynek Bozdech Peter R. Preiser Peter R. Preiser Merozoite invasion properties of <i>P. falciparum</i> W2mef, FCR3, Dd2, 3D7 and HB3 clones. Public Library of Science 2008 3d7 hb3 2008-07-11 02:08:17 Figure https://plos.figshare.com/articles/figure/_Merozoite_invasion_properties_of_P_falciparum_W2mef_FCR3_Dd2_3D7_and_HB3_clones_/597697 <p>(A) Comparison of merozoite invasion inhibition with αrRII-3 and αrtRVIII, including pre-immune serum in 1∶10 and 1∶640 dilutions. Positive control was the invasion of merozoites into the normal complete medium. The error bar denotes the SE. * <i>p</i><0.001, indicating that the invasion of W2mef, FCR3 and Dd2 were successfully inhibited by αrRII-3, compared to 3D7 and HB3. + <i>p</i><0.05 indicating that Dd2 exhibit an intermediate sensitivity to inhibitory antiserum as compared to W2mef and FCR3. (B) Parasites were also assayed for their ability to invade neuraminidase-treated erythrocytes (1mU/ml final). For each parasite clone, the percentage of inhibition of the ability to invade neuraminidase-treated erythrocytes (vertical axis) is plotted against the percentage of invasion inhibition of αrRII-3 in a 1∶10 dilution (horizonal axis). Western blot of enriched culture supernatant from all 5 clones used in this study and probed with αrRII-3, αrtRVIII and αSERA5 was shown in the insert of (B). No RH1 protein was detected in 3D7 and HB3 clones compared with W2mef, Dd2 and FCR3.</p>