10.1371/journal.pone.0193196
Lyvianne Decourtye
Lyvianne
Decourtye
Maud Clemessy
Maud
Clemessy
Erik Mire
Erik
Mire
Tatiana Ledent
Tatiana
Ledent
Laurence Périn
Laurence
Périn
Iain C. Robinson
Iain C.
Robinson
Yves Le Bouc
Yves
Le Bouc
Laurent Kappeler
Laurent
Kappeler
Impact of insulin on primary arcuate neurons culture is dependent on early-postnatal nutritional status and neuronal subpopulation
Public Library of Science
2018
GH secretory capacity
arcuate explants culture
control axonal growth
role
status
IGF -1-mediated effects
Arc neurons
pup
Arc axonal growth
programming
GHRH neurons
insulin
arcuate neurons culture
2018-02-21 18:49:33
Dataset
https://plos.figshare.com/articles/dataset/Impact_of_insulin_on_primary_arcuate_neurons_culture_is_dependent_on_early-postnatal_nutritional_status_and_neuronal_subpopulation/5911882
<div><p>Nutrition plays a critical role in programming and shaping linear growth during early postnatal life through direct action on the development of the neuroendocrine somatotropic (GH/IGF-1) axis. IGF-1 is a key factor in modulating the programming of linear growth during this period. Notably, IGF-1 preferentially stimulates axonal growth of GHRH neurons in the arcuate nucleus of the hypothalamus (Arc), which is crucial for the proliferation of somatotroph progenitors in the pituitary, thus influencing later GH secretory capacity. However, other nutrition-related hormones may also be involved. Among them, insulin shares several structural and functional similarities with IGF-1, as well as downstream signaling effectors. We investigated the role of insulin in the control of Arc axonal growth using an <i>in vitro</i> model of arcuate explants culture and a cell-type specific approach (GHRH-eGFP mice) under both physiological conditions (normally fed pups) and those of dietary restriction (underfed pups). Our data suggest that insulin failed to directly control axonal growth of Arc neurons or influence specific IGF-1-mediated effects on GHRH neurons. Insulin may act on neuronal welfare, which appears to be dependent on neuronal sub-populations and is influenced by the nutritional status of pups in which Arc neurons develop.</p></div>