Cao, Feng A. Wagner, Roger D. Wilson, Kitchener Xie, Xiaoyan Fu, Ji-Dong Drukker, Micha Lee, Andrew A. Li, Ronald S. Gambhir, Sanjiv L. Weissman, Irving C. Robbins, Robert Wu, Joseph C. Electrophysiological recordings of hESC-CMs. <p>Action potential (AP) recordings from single cells were done using the whole-cell patch-clamp technique. hESC-CMs were categorized into pacemaker-, atrial- or ventricular-like phenotypes, based on such common electrophysiological characteristics as the AP amplitude (mV), upstroke velocity (mV/ms), APD50 and APD90 (ms), as well as the resting membrane potential (RMP, mV). (a) Representative ventricular-, atrial- and pacemaker-like action potentials, demonstrating electrophysiological heterogeneity in our hESC-CM population, and (b) Ca2+ transients recorded from hESC-derived cardiomyocytes, confirming calcium influx of these cells. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003474#s4" target="_blank">Materials and Methods</a> for description of experimental parameters.</p> recordings 2008-10-22
    https://plos.figshare.com/articles/figure/_Electrophysiological_recordings_of_hESC_CMs_/589159
10.1371/journal.pone.0003474.g003