Electrophysiological recordings of hESC-CMs. CaoFeng A. WagnerRoger D. WilsonKitchener XieXiaoyan FuJi-Dong DrukkerMicha LeeAndrew A. LiRonald S. GambhirSanjiv L. WeissmanIrving C. RobbinsRobert WuJoseph C. 2008 <p>Action potential (AP) recordings from single cells were done using the whole-cell patch-clamp technique. hESC-CMs were categorized into pacemaker-, atrial- or ventricular-like phenotypes, based on such common electrophysiological characteristics as the AP amplitude (mV), upstroke velocity (mV/ms), APD50 and APD90 (ms), as well as the resting membrane potential (RMP, mV). (a) Representative ventricular-, atrial- and pacemaker-like action potentials, demonstrating electrophysiological heterogeneity in our hESC-CM population, and (b) Ca2+ transients recorded from hESC-derived cardiomyocytes, confirming calcium influx of these cells. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003474#s4" target="_blank">Materials and Methods</a> for description of experimental parameters.</p>