10.1371/journal.pone.0003474.g003
Feng Cao
Feng
Cao
Roger A. Wagner
Roger
A. Wagner
Kitchener D. Wilson
Kitchener
D. Wilson
Xiaoyan Xie
Xiaoyan
Xie
Ji-Dong Fu
Ji-Dong
Fu
Micha Drukker
Micha
Drukker
Andrew Lee
Andrew
Lee
Ronald A. Li
Ronald
A. Li
Sanjiv S. Gambhir
Sanjiv
S. Gambhir
Irving L. Weissman
Irving
L. Weissman
Robert C. Robbins
Robert
C. Robbins
Joseph C. Wu
Joseph C.
Wu
Electrophysiological recordings of hESC-CMs.
Public Library of Science
2008
recordings
2008-10-22 02:32:39
Figure
https://plos.figshare.com/articles/figure/_Electrophysiological_recordings_of_hESC_CMs_/589159
<p>Action potential (AP) recordings from single cells were done using the whole-cell patch-clamp technique. hESC-CMs were categorized into pacemaker-, atrial- or ventricular-like phenotypes, based on such common electrophysiological characteristics as the AP amplitude (mV), upstroke velocity (mV/ms), APD50 and APD90 (ms), as well as the resting membrane potential (RMP, mV). (a) Representative ventricular-, atrial- and pacemaker-like action potentials, demonstrating electrophysiological heterogeneity in our hESC-CM population, and (b) Ca2+ transients recorded from hESC-derived cardiomyocytes, confirming calcium influx of these cells. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003474#s4" target="_blank">Materials and Methods</a> for description of experimental parameters.</p>