10.1371/journal.pone.0003474.g003 Feng Cao Feng Cao Roger A. Wagner Roger A. Wagner Kitchener D. Wilson Kitchener D. Wilson Xiaoyan Xie Xiaoyan Xie Ji-Dong Fu Ji-Dong Fu Micha Drukker Micha Drukker Andrew Lee Andrew Lee Ronald A. Li Ronald A. Li Sanjiv S. Gambhir Sanjiv S. Gambhir Irving L. Weissman Irving L. Weissman Robert C. Robbins Robert C. Robbins Joseph C. Wu Joseph C. Wu Electrophysiological recordings of hESC-CMs. Public Library of Science 2008 recordings 2008-10-22 02:32:39 Figure https://plos.figshare.com/articles/figure/_Electrophysiological_recordings_of_hESC_CMs_/589159 <p>Action potential (AP) recordings from single cells were done using the whole-cell patch-clamp technique. hESC-CMs were categorized into pacemaker-, atrial- or ventricular-like phenotypes, based on such common electrophysiological characteristics as the AP amplitude (mV), upstroke velocity (mV/ms), APD50 and APD90 (ms), as well as the resting membrane potential (RMP, mV). (a) Representative ventricular-, atrial- and pacemaker-like action potentials, demonstrating electrophysiological heterogeneity in our hESC-CM population, and (b) Ca2+ transients recorded from hESC-derived cardiomyocytes, confirming calcium influx of these cells. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003474#s4" target="_blank">Materials and Methods</a> for description of experimental parameters.</p>