Characterization of the scFvF7-Fc-vcMMAE conjugate. Aleksandra Borek Aleksandra Sokolowska-Wedzina Grzegorz Chodaczek Jacek Otlewski 10.1371/journal.pone.0192194.g007 https://plos.figshare.com/articles/figure/Characterization_of_the_scFvF7-Fc-vcMMAE_conjugate_/5870406 <p>(a) SDS-PAGE analysis of scFvF7-Fc and scFvF7-Fc-vcMMAE under reducing conditions. (b-d) Cytotoxicity of scFvF7-Fc-vcMMAE, scFvF7-Fc and MMAE on (b) Snu-16, (c) NCI-H716, and (d) U2OS cells. Cell viability was determined by AlamarBlue Proliferation Assay Kit. Data show average values of triplicate wells ± SD from a representative experiment of two that were performed.</p> 2018-02-08 18:45:39 FGFR 2-positive tumor cells affinity NCI-H scFvF 7-Fc antibody MMAE fragment scFv phage display libraries Tomlinson extracellular ligand-binding domain internalizing anti-FGFR 2 single-chain ECD cytotoxic drug monomethyl-auristatin E FGF receptor 2 bivalent diabody format FGFR 2 cancers Fibroblast growth factor receptors nanomolar K D values scFvF 7-Fc FGFR 2 overexpression phage display technology