Expression of immune-associated genes in splenic myeloid subsets before and after WNV-infection. A. BryanMarianne GiordanoDaniela DravesKevin E. GreenRichard Gale Jr.Michael A. ClarkEdward 2018 <p>F4/80<sup>hi</sup> MΦs, CD11b<sup>-</sup> DCs, CD11b<sup>+</sup> DCs or CD11b<sup>+</sup>SSC<sup>lo</sup> MOs splenic subsets were isolated by cell sorting from either naïve B6 mice or from PBSL-treated mice infected 4 days previously with 1000 pfu WNV/Tx. RNA was isolated, after which expression of 108 immune-associated genes were determined (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191690#sec002" target="_blank">Materials and Methods</a> and Table A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191690#pone.0191690.s001" target="_blank">S1 File</a>). Heat maps show the gene expression/population for the 4 subsets relative to the total gene expression in myeloid cells obtained from (<b>A</b>) naïve mice (mean of 2 independent experiments) or (<b>B</b>) WNV-infected mice (mean of 3 experiments). Ratios were loaded into R statistical programming language (version 3.2.0). Pearson correlation was performed on ratios and displayed in R/Bioconductor using the gplots package. Gene expression/population shown is calculated as expression of the given population X the frequency of the same population in the spleen. Details of expression levels are available in Tables B and C in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0191690#pone.0191690.s001" target="_blank">S1 File</a>.</p>