10.1371/journal.pone.0190333.g003 Miki Shimbo Miki Shimbo Riku Suzuki Riku Suzuki Sayaka Fuseya Sayaka Fuseya Takashi Sato Takashi Sato Katsue Kiyohara Katsue Kiyohara Kozue Hagiwara Kozue Hagiwara Risa Okada Risa Okada Hiromasa Wakui Hiromasa Wakui Yuki Tsunakawa Yuki Tsunakawa Hideto Watanabe Hideto Watanabe Koji Kimata Koji Kimata Hisashi Narimatsu Hisashi Narimatsu Takashi Kudo Takashi Kudo Satoru Takahashi Satoru Takahashi Phenotype of Col2-DKO mice. Public Library of Science 2017 WT Quantitative RT-PCR analysis t 2 t 1 CS synthesis cartilage-specific t 1 t 1 KO mice Col 2a 1 chondrocyte endochondral ossification ECM CS content Ptch 1 transcripts t 2 KO mice chondroitin sulfate N GAG expression levels t 1 KO cartilage -2 Chondroitin sulfate Col 2-DKO mice DKO cartilage t 1 KO E 18.5 cartilage Col 2-DKO knee cartilage 2017-12-29 18:34:03 Figure https://plos.figshare.com/articles/figure/Phenotype_of_Col2-DKO_mice_/5744877 <p>(A) Representative photograph of Control pups and Col2-DKO littermates at E18.5, P7, and P14. Scale bar: 1 cm. (B) Body weight of Control (E18.5; n = 16, P7; n = 4, P14; n = 4) and Col2-DKO (E18.5; n = 12, P7; n = 4, P14; n = 4) mice at various stages. *<i>P</i> < 0.05, **<i>P</i> < 0.01. (C) Humeral and tibial bone length at E18.5 of Control (n = 6) and Col2-DKO (n = 7) mice. (D) Quantitative analysis of <i>t1</i> and <i>t2</i> transcripts in the knee cartilage of WT (n = 3) and Col2-DKO (n = 3) mice at E18.5 using real-time RT-PCR. The expression of each transcript was normalized to that of β-actin. **<i>P</i> < 0.01.</p>