Shimbo, Miki Suzuki, Riku Fuseya, Sayaka Sato, Takashi Kiyohara, Katsue Hagiwara, Kozue Okada, Risa Wakui, Hiromasa Tsunakawa, Yuki Watanabe, Hideto Kimata, Koji Narimatsu, Hisashi Kudo, Takashi Takahashi, Satoru Phenotype of DKO mice. <p>(A) Representative photograph of <i>t2</i> KO pups and cyanotic DKO littermates at E18.5. Scale bar: 1 cm. (B) Body weight of <i>t2</i> KO (n = 68) and DKO (n = 51) embryos at E18.5. *<i>P</i> < 0.05. (C) Lung sections from <i>t2</i> KO embryos and DKO littermates were stained with HE. Scale bar: 100 μm. (D-H) Skeletal whole body (D), upper limb (E), lower limb (F), lumber spine (G), and cranial bone (H) preparation. (I) Humeral and tibial bone length at E18.5 of <i>t2</i> KO (n = 5) and DKO (n = 10) mice. **<i>P</i> < 0.01.</p> WT;Quantitative RT-PCR analysis;t 2;t 1;CS synthesis;cartilage-specific t 1;t 1 KO mice;Col 2a 1;chondrocyte;endochondral ossification;ECM;CS content;Ptch 1 transcripts;t 2 KO mice;chondroitin sulfate N;GAG expression levels;t 1 KO cartilage;-2 Chondroitin sulfate;Col 2-DKO mice;DKO cartilage;t 1 KO;E 18.5 cartilage;Col 2-DKO knee cartilage 2017-12-29
    https://plos.figshare.com/articles/figure/Phenotype_of_DKO_mice_/5744871
10.1371/journal.pone.0190333.g002