Morpholino knockdown of <i>etsrp</i> reduces expression of genes identified by <i>etsrp</i> overexpression microarray analysis. A. GomezGustavo B. VeldmanMatthew ZhaoYan BurgessShawn LinShuo 2009 <p>Embryos were injected with a combination of two <i>etsrp</i> targeting morpholinos (4 ng each) and gene expression was examined by in situ hybridization at 24–26 hours post fertilization. Representative control embryos are on the left of each panel and <i>etsrp</i> morpholino (MO) injected embryos are on the right. (A) <i>krml2</i> is reduced in primitive myeloid cells, posterior cardinal vein, and caudal hematopoietic tail region by <i>etsrp</i> knockdown. (B) <i>lrrp33</i> expression is absent in myeloid cells and reduced in the caudal hematopoietic tail region. (C) <i>hapln1b</i> expression is lost in the posterior cardinal vein when <i>etsrp</i> is knocked down. (D–N) <i>Etsrp</i> knockdown causes a significant decrease of gene expression, most pronounced in the trunk (unlabeled arrows). (D) <i>rasgfp3</i>; (E) <i>tem8</i>; (F) <i>arhgap29</i>; (G) <i>fgd5</i>; (H) <i>yrk</i>; (I) <i>similar to hemicentin</i>; (J) <i>sh3gl3</i>; (K) <i>similar to costimulatory protein</i>; (L) <i>ldb2</i>; (M) <i>est:AI7201944</i>; and (N) <i>est:AW019729</i>. Note that non-hematovascular tissues such as somites in (A) and hypochord in (C) are not affected by <i>etsrp</i> knockdown. Abbreviations: pm, primitive myeloid; pcv, posterior cardinal vein; cht, caudal hematopoietic tail region; ss, somites; and hc, hypochord. Scale bar: 250 µm.</p>