10.1371/journal.pone.0189586.g011 Maëlle Lorvellec Maëlle Lorvellec Federico Scottoni Federico Scottoni Claire Crowley Claire Crowley Rebeca Fiadeiro Rebeca Fiadeiro Panagiotis Maghsoudlou Panagiotis Maghsoudlou Alessandro Filippo Pellegata Alessandro Filippo Pellegata Francesca Mazzacuva Francesca Mazzacuva Asllan Gjinovci Asllan Gjinovci Anne-Marie Lyne Anne-Marie Lyne Justine Zulini Justine Zulini Daniel Little Daniel Little Olukunbi Mosaku Olukunbi Mosaku Deirdre Kelly Deirdre Kelly Paolo De Coppi Paolo De Coppi Paul Gissen Paul Gissen ASGPR expression by hiPSC- or hESC-derived hepatocytes in 2D or 3D on day 13 of differentiation. Public Library of Science 2017 hepatocyte marker albumin 2 D cultures hepatocyte marker alpha-fetoprotein 2 D conditions hepatocyte-like cells Liver transplantation regenerative medicine applications Pluripotent Stem Cells cell hepatocyte differentiation 3 D culture organ regeneration therapies optimised 3 D protocols blood vessel network mouse decellularised liver scaffold 3 D donor organ shortage limits hepatocyte differentiation hiPSC 2017-12-20 18:38:46 Figure https://plos.figshare.com/articles/figure/ASGPR_expression_by_hiPSC-_or_hESC-derived_hepatocytes_in_2D_or_3D_on_day_13_of_differentiation_/5724601 <p>(a-b) Immunostaining of hepatocyte sinusoidal membrane protein ASGPR in 2D (a) and 3D (b) cultured cells. Nuclei stained with DAPI. The higher magnification images of merged channels in the bottom panel correspond to the delineated lower magnification images in the panel above. Images of 3D cultured cells demonstrate membrane localisation of ASGPR.</p>