%0 Figure %A Caccamo, Nadia %A Guggino, Giuliana %A Meraviglia, Serena %A Gelsomino, Giuseppe %A Di Carlo, Paola %A Titone, Lucina %A Bocchino, Marialuisa %A Galati, Domenico %A Matarese, Alessandro %A Nouta, Jan %A R. Klein, Michel %A Salerno, Alfredo %A Sanduzzi, Alessandro %A Dieli, Francesco %A H. M. Ottenhoff, Tom %D 2013 %T Polyfunctional cytokine production analysis of tetramer+ Mtb-specific CD8 T-cells. %U https://plos.figshare.com/articles/figure/_Polyfunctional_cytokine_production_analysis_of_tetramer_Mtb_specific_CD8_T_cells_/568645 %R 10.1371/journal.pone.0005528.g003 %2 https://plos.figshare.com/ndownloader/files/898207 %K cytokine %K mtb-specific %K cd8 %X
Peripheral blood mononuclear cells (PBMC) were stimulated with the same individual peptides as those present in tetramers and were stained with mAbs to CD8, IFN-γ and IL-2, or with isotype-control mAbs. After gating on CD8+ cells, the percentage of cells expressing IFN-γ and IL-2 was determined. (A) Representative intracellular cytokine staining data in one subject with LTBI, one TB patient before therapy and one PPD− healthy donor. Numbers in the corners indicate the percentage of CD8+ cytokine-positive cells in each quadrant. (B) Summary cumulative data of the IFN-γ and IL-2 secretion capability of tetramer+ Mtb-specific CD8 T-cells in LTBI subjects (white bars) and TB patients with active disease before therapy (black bars). The data are expressed as the percentage of CD8+ T-cells that are IFN-γ+/IL-2− or IFN-γ+/IL-2+. The values reported are the mean percentage of the different subset analysed for each group tested ± standard deviations (SD). *p<0.001 and **p<0.01 when compared to values in LTBI subjects.
%I PLOS ONE