Haridas, Valsala Nishimura, Goshi Xu, Zhi-Xiang Connolly, Fiona Hanausek, Margaret Walaszek, Zbigniew Zoltaszek, Robert U. Gutterman, Jordan Avicin D downregulates Stat3-induced protein expression. <p>(A) Effect of avicin D on Stat3 -mediated luciferase gene expression. U266 cells were transfected with Ly6E-Luciferase-Stat3 plasmid as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005578#s4" target="_blank">methods</a>. 24 hrs later cells were washed and treated first with avicin D (1 µM) for 16 hrs, and then exposed to IL-6 (10 ng/ml) for 30 min. Luciferase activity was measured as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005578#s4" target="_blank">methods</a>. The luciferase activity of the treated samples is expressed relative to the activity of untreated control (no avicin, no IL-6), which is taken as 100%. (B) Effect of avicin D on IL-6 production in U266 cells. Cells were treated with 0–1 µM of avicin D for 48 hrs. Cell supernatants were assayed for IL-6 levels using an ELISA kit. (C) Effect of avicin D on the expression of Stat3 regulated proteins. U266 cells were treated with 1 µM of avicin D for 0–24 hrs. Whole cell lysates were analyzed for the expression of different proteins by western blot analysis as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005578#s4" target="_blank">methods</a>.</p> downregulates;stat3-induced 2009-05-18
    https://plos.figshare.com/articles/figure/_Avicin_D_downregulates_Stat3_induced_protein_expression_/568643
10.1371/journal.pone.0005578.g004