Materials used in the mutagenesis process for creating plasmids with two Lac repressor binding sites. HanLin G. GarciaHernan BlumbergSeth TowlesKevin B. F. BeausangJohn C. NelsonPhilip PhillipsRob 2009 <p>Primer sequences(5′→3′):</p><p>Mut0: ctaactcacattaattgcgttgAgctcGAGgTTcgctttccagtc.</p><p>Mut1: catacgagccggaa (G) cataaagtgtaaagc.</p><p>Mut2: ctcggaaagaaca AATTGTGAGCGCTCACAATT aaggccaggaacc.</p><p>Mut3: ctcggaaagaacat AATTGTGAGCGCTCACAATT aggccaggaaccg.</p><p>Mut4: cggaaagaacatg AATTGTGAGCGCTCACAATT ggccaggaaccgt.</p><p>Mut5: ggaaagaacatgt AATTGTGAGCGCTCACAATT gccaggaaccgta.</p><p>Mut6: gaaagaacatgtg AATTGTGAGCGCTCACAATT ccaggaaccgtaa.</p><p>Mut7: cggaaagaacatgtga AATTGTGAGCGCTCACAATT caggaaccgtaaaaag.</p><p>Mut8: ggaaagaacatgtgag AATTGTGAGCGCTCACAATT aggaaccgtaaaaagg.</p><p>Mut9: gaaagaacatgtgagc AATTGTGAGCGCTCACAATT ggaaccgtaaaaaggc.</p><p>Mut10: catacgagccggaag [C] cataaagtgtaaagc.</p><p>Mut11: catacgagccggaag [CG] cataaagtgtaaagc.</p><p>The capital letters in the primer sequences indicate the mutations. ‘()’ indicates bp deletion and ‘[ ]’ indicates bp addition. The inter-operator distance indicated here is the distance between two inner edges of the operators instead of center to center distance that is commonly used in <i>in vivo</i> experiments <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005621#pone.0005621-Mller1" target="_blank">[11]</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005621#pone.0005621-Becker2" target="_blank">[13]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005621#pone.0005621-Oehler1" target="_blank">[86]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005621#pone.0005621-Oehler2" target="_blank">[93]</a>.</p>