Kuboyama, Kazuya Tanga, Naomi Suzuki, Ryoko Fujikawa, Akihiro Noda, Masaharu Cell-based phenotypic assays using OL1 cells. <p>Double-immunofluorescence labeling of OL1 cells (upper). OL1 cells were cultured with differentiation medium containing thyroid hormones on dishes coated with poly-<i>L</i>-ornithine with/without aggrecan at the indicated concentrations. After 10 days, cells were fixed with formalin and stained with anti-NG2 proteoglycan (OPCs; <i>red</i>) and anti-MBP (mature oligodendrocytes; <i>green</i>) antibodies, in conjunction with the 4′,6-diamidino-2-phenylindole (DAPI) labeling of nuclei (<i>blue</i>). Scale bars, 100 μm. Scatter plots show the ratio of MBP-positive cells to NG2-positive cells (lower left), and total DAPI-positive cell numbers relative to the average of the vehicle control (lower right). Each circle corresponds to an independent cell culture (<i>n</i> = 5 each). *, <i>p</i> < 0.05 and **, <i>p</i> < 0.01, significant difference between the indicated groups (analysis of variance with Bonferroni’s <i>post-hoc</i> tests).</p> oligodendrocyte differentiation Chondroitin sulfate proteoglycans;PRM;protein tyrosine phosphatase receptor type Z;CSPG;chondroitin sulfate proteoglycan-mediated inhibition;MS;mouse OPC-like OL 1 cells;oligodendrocyte precursor cells;oligodendrocyte differentiation;CNS;PTPRZ 2017-12-07
    https://plos.figshare.com/articles/figure/Cell-based_phenotypic_assays_using_OL1_cells_/5680057
10.1371/journal.pone.0189164.g002