Flow cytometry analyses of keratinocyte basal and differentiated populations bound by TAMRA and EM-TAMRA.
Cheryl Leong
Christine Neumann
Srinivas Ramasamy
Bhimsen Rout
Lim Yi Wee
Mei Bigliardi-Qi
Paul L. Bigliardi
10.1371/journal.pone.0188607.g003
https://plos.figshare.com/articles/figure/Flow_cytometry_analyses_of_keratinocyte_basal_and_differentiated_populations_bound_by_TAMRA_and_EM-TAMRA_/5676973
<p>EM-TAMRA labeling of both basal and differentiated keratinocytes were performed in 4°C at a dose of 500 nM. The labeled cells were subjected to population based flow cytometry analysis to study basal keratinocytes (A and C) and differentiated keratinocytes (B and D) using TAMRA dye alone (A and B) and EM-TAMRA (C and D). Representative images in (C) and (D) show specific separation of sub-population in EM-TAMRA labeled basal and differentiated keratinocytes distinct from TAMRA dye alone. Specific population was observed with respective percentages for both basal (8.3%) and differentiated (22.2%) cell types as represented in Table E. Data shown are calculated from six independent biological replicates (N = 6) and represent mean ± SD for respective concentrations (10 nM – 2 μM).</p>
2017-12-06 18:41:31
mechanism
μ- ORs
skin cells
ligand Opioids
EM-TAMRA -μ-OR interactions
μ- OR-Endomorphine complexes
flow cytometry
antagonist treatments
opioid agonist
stress response
keratinocyte
non-canonical effects
skin conditions
ligand-receptor interactions
Naltrexone
confocal microscopy
ligand Endomorphine
skin function
increases EM-TAMRA binding
CTOP show
novel treatment strategies
offer opportunities
population analysis
dye Tetramethylrhodamine