%0 Figure %A V. Correia, Daniel %A d'Orey, Francisco %A A. Cardoso, Bruno %A Lança, Telma %A R. Grosso, Ana %A deBarros, Ana %A R. Martins, Leila %A T. Barata, João %A Silva-Santos, Bruno %D 2009 %T HMB-PP does not induce down-modulation of surface Vγ9+ TCR and sustains cytokine production upon re-stimulation. %U https://plos.figshare.com/articles/figure/_HMB_PP_does_not_induce_down_modulation_of_surface_V_9_TCR_and_sustains_cytokine_production_upon_re_stimulation_/567662 %R 10.1371/journal.pone.0005657.g005 %2 https://plos.figshare.com/ndownloader/files/897218 %K induce %K down-modulation %K tcr %K sustains %K cytokine %X

(A) MACS-sorted γδ PBL (of which 80–90% Vγ9+) were incubated for the indicated times with HMB-PP or OKT3, and stained with anti-Vγ9 mAb for flow cytometry analysis. Bold lines represent treated cells, while shaded are non-stimulated Vγ9+ cells (time = 0 hrs). (B) Confocal microscopy photos of γδ T-cells cultured for 24 hrs as in (A) and then stained for Vγ9+ TCR. (C–D) Experimental design (C) and CBA analysis (D) of the re-stimulation response of MACS-sorted γδ PBL. After 36 hrs of stimulation, cells were re-plated for secondary activation during 24 hrs, when supernatants were collected and analyzed for Th1 cytokines by CBA. Error bars represent SD and differences refer to IL-2 controls (ns, non-significant; **p<0.01; ***p<0.001). Results shown in this figure are representative of 2–5 independent experiments.

%I PLOS ONE