DENV RNA replication induces IL-27 expression in dermal CD14<sup>+</sup> DCs. Joris K. Sprokholt Tanja M. Kaptein John L. van Hamme Ronald J. Overmars Sonja I. Gringhuis Teunis B. H. Geijtenbeek 10.1371/journal.ppat.1006738.g007 https://plos.figshare.com/articles/figure/DENV_RNA_replication_induces_IL-27_expression_in_dermal_CD14_sup_sup_DCs_/5647879 <p>(A) CD14<sup>+</sup> and CD1c<sup>+</sup> dermal DCs were isolated from human skin and mock-treated or infected with DENV for indicated times before mRNA expression of IFN-β, MxA and IL-27p28 was analyzed using real-time PCR, normalized to GAPDH and set at 1 in 32h infected samples of CD14<sup>+</sup> dermal DCs. (B) Similar to (A), but in the presence or absence of DENV replication inhibitor SDM25N. Data are collated (mean ± s.d.) of two independent experiments with different donors. ** <i>P</i><0.01, * <i>P</i><0.05 (student’s t-test). N.S., not significant; N.D. not detected.</p> 2017-11-29 18:39:01 drive IL -27 production dengue virus drives RIG-I-like receptor activation STAT IFN PD T FH drives T FH formation T helper cell formation T FH induction antibody production Follicular T helper cells MDA T FH cells IKK T FH formation infection ISGF 3. Inhibiting RLR activation DC DENV-induced CXCR 5 IL -27 secretion T FH polarization