DENV RNA replication induces IL-27 expression in dermal CD14<sup>+</sup> DCs.
Joris K. Sprokholt
Tanja M. Kaptein
John L. van Hamme
Ronald J. Overmars
Sonja I. Gringhuis
Teunis B. H. Geijtenbeek
10.1371/journal.ppat.1006738.g007
https://plos.figshare.com/articles/figure/DENV_RNA_replication_induces_IL-27_expression_in_dermal_CD14_sup_sup_DCs_/5647879
<p>(A) CD14<sup>+</sup> and CD1c<sup>+</sup> dermal DCs were isolated from human skin and mock-treated or infected with DENV for indicated times before mRNA expression of IFN-β, MxA and IL-27p28 was analyzed using real-time PCR, normalized to GAPDH and set at 1 in 32h infected samples of CD14<sup>+</sup> dermal DCs. (B) Similar to (A), but in the presence or absence of DENV replication inhibitor SDM25N. Data are collated (mean ± s.d.) of two independent experiments with different donors. ** <i>P</i><0.01, * <i>P</i><0.05 (student’s t-test). N.S., not significant; N.D. not detected.</p>
2017-11-29 18:39:01
drive IL -27 production
dengue virus drives
RIG-I-like receptor activation
STAT
IFN
PD
T FH
drives T FH formation
T helper cell formation
T FH induction
antibody production Follicular T helper cells
MDA
T FH cells
IKK
T FH formation
infection
ISGF 3. Inhibiting RLR activation
DC
DENV-induced CXCR 5
IL -27 secretion
T FH polarization