WB analyses of synaptophysin in the cortex of TG and WT mice 9 months post-irradiation. Emil Rudobeck John A. Bellone Attila Szücs Kristine Bonnick Shalini Mehrotra-Carter Jerome Badaut Gregory A. Nelson Richard E. Hartman Roman Vlkolinský 10.1371/journal.pone.0186168.g008 https://plos.figshare.com/articles/figure/WB_analyses_of_synaptophysin_in_the_cortex_of_TG_and_WT_mice_9_months_post-irradiation_/5645959 <p>The average synaptophysin levels in TG control mice were significantly elevated when compared to WT controls. Irradiation of TG mice at 0.5 and 1 Gy had no effect on synaptophysin levels. In WT mice, irradiation significantly elevated synaptophysin to levels comparable to those observed in TG groups. Insets: Original images of WB gels prepared from TG (top panel) and WT (bottom panel) mice. Immuno-reactive bands optical densities indicate levels of synaptophysin (~38 kDa) and β-tubulin (~50 kDa) in cortical homogenates of control and irradiated 0.5 Gy mice. TG mice: N = 6, 5, 5 animals/radiation group; WT mice: N = 8, 8 animals/radiation group. Statistical analyses: 2-way ANOVA, Post hoc *** <i>p</i><0.001 WT 0 Gy vs 0.5 Gy; †† <i>p</i><0.01 WT vs TG controls. Data represent means ± SEM.</p> 2017-11-29 18:23:17 CCL 9 months post-irradiation WT mice WM Low-dose proton radiation effects space travel Space radiation Ground-based animal studies APP synaptic transmission TG mice presynaptic marker synaptophysin CXCL bead-based multiplex assay space radiation spectrum β deposition 6 months post-irradiation CA 1 neurons AD TNF IL pro-inflammatory cytokine levels