WB analyses of synaptophysin in the cortex of TG and WT mice 9 months post-irradiation.
Emil Rudobeck
John A. Bellone
Attila Szücs
Kristine Bonnick
Shalini Mehrotra-Carter
Jerome Badaut
Gregory A. Nelson
Richard E. Hartman
Roman Vlkolinský
10.1371/journal.pone.0186168.g008
https://plos.figshare.com/articles/figure/WB_analyses_of_synaptophysin_in_the_cortex_of_TG_and_WT_mice_9_months_post-irradiation_/5645959
<p>The average synaptophysin levels in TG control mice were significantly elevated when compared to WT controls. Irradiation of TG mice at 0.5 and 1 Gy had no effect on synaptophysin levels. In WT mice, irradiation significantly elevated synaptophysin to levels comparable to those observed in TG groups. Insets: Original images of WB gels prepared from TG (top panel) and WT (bottom panel) mice. Immuno-reactive bands optical densities indicate levels of synaptophysin (~38 kDa) and β-tubulin (~50 kDa) in cortical homogenates of control and irradiated 0.5 Gy mice. TG mice: N = 6, 5, 5 animals/radiation group; WT mice: N = 8, 8 animals/radiation group. Statistical analyses: 2-way ANOVA, Post hoc *** <i>p</i><0.001 WT 0 Gy vs 0.5 Gy; †† <i>p</i><0.01 WT vs TG controls. Data represent means ± SEM.</p>
2017-11-29 18:23:17
CCL
9 months post-irradiation
WT mice
WM
Low-dose proton radiation effects
space travel Space radiation
Ground-based animal studies
APP
synaptic transmission
TG mice
presynaptic marker synaptophysin
CXCL
bead-based multiplex assay
space radiation spectrum
β deposition
6 months post-irradiation
CA 1 neurons
AD
TNF
IL
pro-inflammatory cytokine levels